Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/113597
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dc.contributor.authorKalko, Susana-
dc.contributor.authorPaco Mercader, Sonia-
dc.contributor.authorJou, Cristina-
dc.contributor.authorRodríguez, María Angeles-
dc.contributor.authorMeznaric, Marija-
dc.contributor.authorRogac, Mihael-
dc.contributor.authorJekovec-Vrhovsek, Maja-
dc.contributor.authorSciacco, Monica-
dc.contributor.authorMoggio, Maurizio-
dc.contributor.authorFagiolari, Gigliola-
dc.contributor.authorDe Paepe, Boel-
dc.contributor.authorDe Meirleir, Linda-
dc.contributor.authorFerrer, Isidro (Ferrer Abizanda)-
dc.contributor.authorRoig Quilis, Manuel-
dc.contributor.authorMunell Casadesús, Francina-
dc.contributor.authorMontoya, Julio-
dc.contributor.authorLópez Gallardo, Ester-
dc.contributor.authorRuiz Pesini, Eduardo-
dc.contributor.authorArtuch Iriberri, Rafael-
dc.contributor.authorMontero Sánchez, Raquel-
dc.contributor.authorTorner Rubies, Ferran-
dc.contributor.authorNascimento, Andrés-
dc.contributor.authorOrtez, Carlos Ignacio-
dc.contributor.authorColomer Oferil, Jaume-
dc.contributor.authorJiménez Mallebrera, Cecilia-
dc.date.accessioned2017-07-10T11:53:24Z-
dc.date.available2017-07-10T11:53:24Z-
dc.date.issued2014-02-01-
dc.identifier.issn1471-2164-
dc.identifier.urihttp://hdl.handle.net/2445/113597-
dc.description.abstractBACKGROUND: Mutations in the gene encoding thymidine kinase 2 (TK2) result in the myopathic form of mitochondrial DNA depletion syndrome which is a mitochondrial encephalomyopathy presenting in children. In order to unveil some of the mechanisms involved in this pathology and to identify potential biomarkers and therapeutic targets we have investigated the gene expression profile of human skeletal muscle deficient for TK2 using cDNA microarrays. RESULTS: We have analysed the whole transcriptome of skeletal muscle from patients with TK2 mutations and compared it to normal muscle and to muscle from patients with other mitochondrial myopathies. We have identified a set of over 700 genes which are differentially expressed in TK2 deficient muscle. Bioinformatics analysis reveals important changes in muscle metabolism, in particular, in glucose and glycogen utilisation, and activation of the starvation response which affects aminoacid and lipid metabolism. We have identified those transcriptional regulators which are likely to be responsible for the observed changes in gene expression. CONCLUSION: Our data point towards the tumor suppressor p53 as the regulator at the centre of a network of genes which are responsible for a coordinated response to TK2 mutations which involves inflammation, activation of muscle cell death by apoptosis and induction of growth and differentiation factor 15 (GDF-15) in muscle and serum. We propose that GDF-15 may represent a potential novel biomarker for mitochondrial dysfunction although further studies are required.-
dc.format.extent22 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherBioMed Central-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1186/1471-2164-15-91-
dc.relation.ispartofBmc Genomics, 2014, num. 15, p. 91-
dc.relation.urihttps://doi.org/10.1186/1471-2164-15-91-
dc.rightscc-by (c) Kalko, Susana et al., 2014-
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es-
dc.sourceArticles publicats en revistes (Patologia i Terapèutica Experimental)-
dc.subject.classificationExpressió gènica-
dc.subject.classificationBioinformàtica-
dc.subject.classificationMicroxips d'ADN-
dc.subject.classificationADN mitocondrial-
dc.subject.classificationApoptosi-
dc.subject.classificationMalalties del sistema nerviós central-
dc.subject.classificationInfants-
dc.subject.otherGene expression-
dc.subject.otherBioinformatics-
dc.subject.otherDNA microarrays-
dc.subject.otherMitochondrial DNA-
dc.subject.otherApoptosis-
dc.subject.otherCentral nervous system diseases-
dc.subject.otherChildren-
dc.titleTranscriptomic profiling of TK2 deficient human skeletal muscle suggests a role for the p53 signalling pathway and identifies growth and differentiation factor-15 as a potential novel biomarker for mitochondrial myopathies-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec647634-
dc.date.updated2017-07-10T11:53:24Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid24484525-
Appears in Collections:Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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