Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/114226
Title: The ins and outs of the BCCAo model for chronic hypoperfusion: a multimodal and longitudinal MRI approach
Author: Soria, Guadalupe
Tudela Fernández, Raúl
Márquez-Martín, Ana
Camón, Lluïsa
Batallé Bolaño, Dafnis
Muñoz-Moreno, Emma
Eixarch Roca, Elisenda
Puig, Josep
Pedraza, S.
Vila i Calsina, Elisabet
Prats Galino, Alberto
Planas Obradors, Anna Maria
Keywords: Ressonància magnètica
Artèries cerebrals
Sistema nerviós central
Histologia
Lesions cerebrals
Magnetic resonance
Cerebral arteries
Central nervous system
Histology
Brain damage
Issue Date: 18-Sep-2013
Publisher: Public Library of Science (PLoS)
Abstract: Cerebral hypoperfusion induced by bilateral common carotid artery occlusion (BCCAo) in rodents has been proposed as an experimental model of white matter damage and vascular dementia. However, the histopathological and behavioral alterations reported in this model are variable and a full characterization of the dynamic alterations is not available. Here we implemented a longitudinal multimodal magnetic resonance imaging (MRI) design, including time- of-flight angiography, high resolution T1-weighted images, T2 relaxometry mapping, diffusion tensor imaging, and cerebral blood flow measurements up to 12 weeks after BCCAo or sham-operation in Wistar rats. Changes in MRI were related to behavioral performance in executive function tasks and histopathological alterations in the same animals. MRI frequently (70%) showed various degrees of acute ischemic lesions, ranging from very small to large subcortical infarctions. Independently, delayed MRI changes were also apparent. The patterns of MRI alterations were related to either ischemic necrosis or gliosis. Progressive microstructural changes revealed by diffusion tensor imaging in white matter were confirmed by observation of myelinated fiber degeneration, including severe optic tract degeneration. The latter interfered with the visually cued learning paradigms used to test executive functions. Independently of brain damage, BCCAo induced progressive arteriogenesis in the vertebrobasilar tree, a process that was associated with blood flow recovery after 12 weeks. The structural alterations found in the basilar artery were compatible with compensatory adaptive changes driven by shear stress. In summary, BCCAo in rats induces specific signatures in multimodal MRI that are compatible with various types of histological lesion and with marked adaptive arteriogenesis.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0074631
It is part of: PLoS One, 2013, vol. 8, num. 9, p. e74631
Related resource: https://doi.org/10.1371/journal.pone.0074631
URI: http://hdl.handle.net/2445/114226
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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