Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/114916
Title: | Alzheimer´s Disease-associated Aβ42 Peptide: Expression and Purification for NMR Structural Studies |
Author: | Serra Batiste, Montserrat García Castellanos, Raquel Ninot Pedrosa, Martí Serra Vidal, Bernat Berrow, Nicholas Simon Carulla Casanovas, Natàlia |
Keywords: | Malaltia d'Alzheimer Alzheimer's disease |
Issue Date: | 1-Jul-2017 |
Abstract: | Background: The aggregation of the amyloid-beta peptide (Aβ) in the brain is strongly associated with Alzheimer´s disease (AD). However, the heterogeneous and transient nature of this process has prevented identification of the exact molecular form of Aβ responsible for the neurotoxicity observed in this disease. Therefore, characterizing Aβ aggregation is of utmost importance in the field of AD. Nuclear magnetic resonance spectroscopy (NMR) is a technique that holds great potential to achieve this goal. However, it requires the use of specific labels introduced through recombinant expression of Aβ. Objective: In this paper, we report on a straightforward expression and purification protocol to obtain [U-15N] and [U-2H,13C,15N] Aβ42. Method: Aβ42 is expressed fused to Small Ubiquitin-like Modifier (SUMO) protein, which prevents Aβ42 aggregation. Results: The solubilizing capacity of SUMO has allowed us to design a purification protocol involving immobilized metal affinity chromatography (IMAC), a desalting step, and two size exclusion chromatography (SEC) purifications. Conclusion: This approach, which does not require the use of costly and time-consuming reversed phase high performance liquid chromatography (RP-HPLC), offers a much straightforward strategy to those previously described to obtain [U-15N] Aβ42 and it is the first protocol through which to achieve [U-2H,13C,15N] Aβ42. The peptides obtained are of high purity and have the required isotope enrichment to support NMR-based structural studies. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.2174/2212796811666170206113722 |
It is part of: | Current Chemical Biology, 2017 |
URI: | http://hdl.handle.net/2445/114916 |
Related resource: | http://dx.doi.org/10.2174/2212796811666170206113722 |
ISSN: | 2212-7968 |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.