Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/115631
Title: | Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers |
Author: | Forget, Jeremy Awaja, Firas Gugutkov, Dencho Gustavsson, Juhan Gallego Ferrer, Gloria Coelho-Sampaio, Tatiana Hochman-Mendez, Camila Salmeron-Sanchez, Manuel Altankov, George |
Keywords: | Cèl·lules mare Stem cells Cartílag Cartilage |
Issue Date: | 8-Jun-2016 |
Publisher: | Wiley-VCH Verlag |
Abstract: | Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly-lactic acid nanofibers compared to 3D sandwich-like environments. The adhering cells show well-developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription-polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1002/mabi.201600080 |
It is part of: | Macromolecular Bioscience, 2016, vol. 16, num. 9, p. 1348-1359 |
URI: | http://hdl.handle.net/2445/115631 |
Related resource: | http://dx.doi.org/10.1002/mabi.201600080 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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L02_2016_Macromolecular Bioscience_16_1348_OA.pdf | 5.12 MB | Adobe PDF | View/Open |
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