Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/116224
Title: Chronic Exposure to Malaria Is Associated with Inhibitory and Activation Markers on Atypical Memory B Cells and Marginal Zone-Like B Cells
Author: Ubillos, Itziar
Campo, Joseph J.
Requena, Pilar
Ome-Kaius, Maria
Hanieh, Sarah
Rose, Honor
Samol, Paula
Barrios, Diana
Jiménez, Alfons
Bardají, Azucena
Mueller, Ivo
Menéndez, Clara
Rogerson, Stephen John
Moncunill, Gemma
Dobaño, Carlota, 1969-
Keywords: Malària
Infeccions
Malaria
Infections
Issue Date: 21-Aug-2017
Publisher: Frontiers Media
Abstract: In persistent infections that are accompanied by chronic immune activation, such as human immunodeficiency virus, hepatitis C virus, and malaria, there is an increased frequency of a phenotypically distinct subset of memory B cells lacking the classic memory marker CD27 and showing a reduced capacity to produce antibodies. However, critical knowledge gaps remain on specific B cell changes and immune adaptation in chronic infections. We hypothesized that expansion of atypical memory B cells (aMBCs) and reduction of activated peripheral marginal zone (MZ)-like B cells in constantly exposed individuals might be accompanied by phenotypic changes that would confer a tolerogenic profile, helping to establish tolerance to infections. To better understand malaria-associated phenotypic abnormalities on B cells, we analyzed peripheral blood mononuclear cells from 55 pregnant women living in a malaria-endemic area of Papua Nueva Guinea and 9 Spanish malaria-naïve individuals using four 11-color flow cytometry panels. We assessed the expression of markers of B cell specificity (IgG and IgM), activation (CD40, CD80, CD86, b220, TACI, and CD150), inhibition (PD1, CD95, and CD71), and migration (CCR3, CXCR3, and CD62l). We found higher frequencies of active and resting aMBC and marked reduction of MZ-like B cells, although changes in absolute cell counts could not be assessed. Highly exposed women had higher PD1+-, CD95+-, CD40+-, CD71+-, and CD80+-activated aMBC frequencies than non-exposed subjects. Malaria exposure increased frequencies of b220 and proapoptotic markers PD1 and CD95, and decreased expression of the activation marker TACI on MZ-like B cells. The increased frequencies of inhibitory and apoptotic markers on activated aMBCs and MZ-like B cells in malaria-exposed adults suggest an immune-homeostatic mechanism for maintaining B cell development and function while simultaneously downregulating hyperreactive B cells. This mechanism would keep the B cell activation threshold high enough to control infection but impaired enough to tolerate it, preventing systemic inflammation.
Note: Reproducció del document publicat a: http://dx.doi.org/10.3389/fimmu.2017.00966
It is part of: Frontiers in Immunology, 2017, vol. 8, num.966
URI: http://hdl.handle.net/2445/116224
Related resource: http://dx.doi.org/10.3389/fimmu.2017.00966
ISSN: 1664-3224
Appears in Collections:Articles publicats en revistes (ISGlobal)
Publicacions de projectes de recerca finançats per la UE

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