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Title: | Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
Author: | Gupta, Himanshu Matambisso, Gloria Galatas, Beatriz Cisteró, Pau Nhamussua, Lidia Simone, Wilson Cunningham, Jane Rabinovich, Regina Alonso, Pedro Saute, Francisco Aide, Pedro Carlos Paulino Mayor Aparicio, Alfredo Gabriel |
Keywords: | Malària Moçambic Malaria Mozambique |
Issue Date: | 16-Oct-2017 |
Publisher: | BioMed Central |
Abstract: | BACKGROUND: Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. METHODS: Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. RESULTS: Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)]. CONCLUSIONS: The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-017-2061-z |
It is part of: | Malaria Journal, 2017, vol. 16, num. 1, p. 416 |
URI: | http://hdl.handle.net/2445/117493 |
Related resource: | http://dx.doi.org/10.1186/s12936-017-2061-z |
ISSN: | 1475-2875 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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