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Title: Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique
Author: Gupta, Himanshu
Matambisso, Gloria
Galatas, Beatriz
Cisteró, Pau
Nhamussua, Lidia
Simone, Wilson
Cunningham, Jane
Rabinovich, Regina
Alonso, Pedro
Saute, Francisco
Aide, Pedro Carlos Paulino
Mayor Aparicio, Alfredo Gabriel
Keywords: Malària
Issue Date: 16-Oct-2017
Publisher: BioMed Central
Abstract: BACKGROUND: Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. METHODS: Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. RESULTS: Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)]. CONCLUSIONS: The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required.
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It is part of: Malaria Journal, 2017, vol. 16, num. 1, p. 416
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ISSN: 1475-2875
Appears in Collections:Articles publicats en revistes (ISGlobal)

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