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Title: | The relevance of the UPS in the fatty liver graft preservation: a new approach for IGL-1 and HTK solutions |
Author: | Panisello Roselló, Arnau Verde, Eva Amine Zaouali, Mohamed Flores, Marta Alva Bocanegra, Norma V. (Norma Violeta) Lopez, Alexandre Folch i Puy, Emma Carbonell i Camós, Teresa Hotter Corripio, Georgina Adam, R. (René) Roselló Catafau, Juan |
Keywords: | Isquèmia Ubiqüitina Malalties del fetge Proteïnes Ischemia Ubiquitin Liver diseases Proteins |
Issue Date: | 31-Oct-2017 |
Publisher: | MDPI |
Abstract: | The 26S proteasome is the central proteolytic machinery of the ubiquitin proteasome system (UPS), which is involved in the degradation of ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor in fatty liver graft preservation during organ cold storage using University of Wisconsin solution (UW) and Institute Georges Lopez (IGL-1) solutions. However, the merits of IGL-1 and histidine-tryptophan-ketoglutarate (HTK) solutions for fatty liver preservation have not been compared. Fatty liver grafts from obese Zücker rats were preserved for 24 h at 4 °C. Aspartate aminotransferase and alanine aminotransferase (AST/ALT), glutamate dehydrogenase (GLDH), ATP, adenosine monophosphate protein kinase (AMPK), e-NOS, proteasome activity and liver polyubiquitinated proteins were determined. IGL-1 solution prevented ATP breakdown during cold-storage preservation of steatotic livers to a greater extent than HTK solution. There were concomitant increases in AMPK activation, e-NOS (endothelial NOS (NO synthase)) expression and UPS inhibition. UPS activity is closely related to the composition of the solution used to preserve the organ. IGL-1 solution provided significantly better protection against ischemia-reperfusion for cold-stored fatty liver grafts than HTK solution. The effect is exerted through the activation of the protective AMPK signaling pathway, an increase in e-NOS expression and a dysregulation of the UPS. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms18112287 |
It is part of: | International Journal of Molecular Sciences, 2017, vol. 18, num. 11, p. E2287 |
URI: | http://hdl.handle.net/2445/117629 |
Related resource: | https://doi.org/10.3390/ijms18112287 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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