Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/117632
Title: | Prevalent sequences in the Human Genome Can Form Mini i-Motif Structures at Physiological pH |
Author: | Mir Morro, Bartomeu Serrano, Israel Buitrago Ospina, Diana Camila Orozco López, Modesto Escaja Sánchez, Nuria González, Carlos |
Keywords: | Oligonucleòtids Cromosomes humans Genètica humana Oligonucleotides Human chromosomes Human genetics |
Issue Date: | 21-Sep-2017 |
Publisher: | American Chemical Society |
Abstract: | We report here the solution structure of several repetitive DNA sequences containing d(TCGTTCCGT) and related repeats. At physiological pH, these sequences fold into i-motif like quadruplexes in which every two repeats a globular structure is stabilized by two hemiprotonated C:C+ base pairs, flanked by two minor groove tetrads resulting from the association of G:C or G:T base pairs. The interaction between the minor groove tetrads and the nearby C:C+ base pairs affords a strong stabilization, which results in effective pHT values above 7.5. Longer sequences with more than two repeats are able to fold in tandem, forming a rosary bead-like structure. Bioinformatics analysis shows that these sequences are prevalent in the human genome, and are present in development-related genes. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1021/jacs.7b07383 |
It is part of: | Journal of the American Chemical Society, 2017, vol. 139, num. 40, p. 13985-13988 |
URI: | http://hdl.handle.net/2445/117632 |
Related resource: | https://doi.org/10.1021/jacs.7b07383 |
ISSN: | 0002-7863 |
Appears in Collections: | Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Química Inorgànica i Orgànica) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
673637.pdf | 11.94 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.