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Title: Prevalent sequences in the Human Genome Can Form Mini i-Motif Structures at Physiological pH
Author: Mir Morro, Bartomeu
Serrano, Israel
Buitrago, Diana
Orozco López, Modesto
Escaja Sánchez, Nuria
González, Carlos
Keywords: Oligonucleòtids
Cromosomes humans
Genètica humana
Human chromosomes
Human genetics
Issue Date: 21-Sep-2017
Publisher: American Chemical Society
Abstract: We report here the solution structure of several repetitive DNA sequences containing d(TCGTTCCGT) and related repeats. At physiological pH, these sequences fold into i-motif like quadruplexes in which every two repeats a globular structure is stabilized by two hemiprotonated C:C+ base pairs, flanked by two minor groove tetrads resulting from the association of G:C or G:T base pairs. The interaction between the minor groove tetrads and the nearby C:C+ base pairs affords a strong stabilization, which results in effective pHT values above 7.5. Longer sequences with more than two repeats are able to fold in tandem, forming a rosary bead-like structure. Bioinformatics analysis shows that these sequences are prevalent in the human genome, and are present in development-related genes.
Note: Versió postprint del document publicat a:
It is part of: Journal of the American Chemical Society, 2017, vol. 139, num. 40, p. 13985-13988
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ISSN: 0002-7863
Appears in Collections:Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Química Inorgànica i Orgànica)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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