Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/119377
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Viñas Muñoz, José Luis | - |
dc.contributor.author | Ventayol Espinazo, Marina | - |
dc.contributor.author | Brüne, Bernhard, 1957- | - |
dc.contributor.author | Jung, Michaela | - |
dc.contributor.author | Sola Martínez, Anna | - |
dc.contributor.author | Pi i Siqués, Felip | - |
dc.contributor.author | Mastora, Chrysoula | - |
dc.contributor.author | Hotter Corripio, Georgina | - |
dc.date.accessioned | 2018-01-29T12:38:35Z | - |
dc.date.available | 2018-01-29T12:38:35Z | - |
dc.date.issued | 2013-04-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/119377 | - |
dc.description.abstract | This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0060937 | - |
dc.relation.ispartof | PLoS One, 2013, vol. 8, num. 4, p. e60937 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0060937 | - |
dc.rights | cc-by (c) Viñas Muñoz, José Luis et al., 2013 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Cèl·lules mare embrionàries | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.classification | Diferenciació cel·lular | - |
dc.subject.other | Embryonic stem cells | - |
dc.subject.other | MicroRNAs | - |
dc.subject.other | Cell diferentiation | - |
dc.title | miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 621721 | - |
dc.date.updated | 2018-01-29T12:38:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23593353 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
621721.pdf | 1.31 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License