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Title: Systemic inflammatory response to smoking in chronic obstructive pulmonary disease: evidence of a gender effect
Author: Faner, Rosa
Gonzalez, Núria
Cruz, Tamara
Kalko, Susana
Agustí García-Navarro, Àlvar
Keywords: Malalties pulmonars obstructives cròniques
Hàbit de fumar
Marcadors genètics
Regulació genètica
Chronic obstructive pulmonary diseases
Tobbacco habit
Genetic markers
Genetic regulation
Issue Date: 15-May-2014
Publisher: Public Library of Science (PLoS)
Abstract: Background Tobacco smoking is the main risk factor of chronic obstructive pulmonary disease (COPD) but not all smokers develop the disease. An abnormal pulmonary and systemic inflammatory response to smoking is thought to play a major pathogenic role in COPD, but this has never been tested directly. Methods We studied the systemic biomarker and leukocyte transcriptomic response (Affymetrix microarrays) to smoking exposure in 10 smokers with COPD and 10 smokers with normal spirometry. We also studied 10 healthy never smokers (not exposed to smoking) as controls. Because some aspects of COPD may differ in males and females, and the inflammatory response to other stressors (infection) might be different in man and women, we stratified participant recruitment by sex. Differentially expressed genes were validated by q-PCR. Ontology enrichment was evaluated and interaction networks inferred. Results Principal component analysis identified sex differences in the leukocyte transcriptomic response to acute smoking. In both genders, we identified genes that were differentially expressed in response to smoking exclusively in COPD patients (COPD related signature) or smokers with normal spirometry (Smoking related signature), their ontologies and interaction networks. Conclusions The use of an experimental intervention (smoking exposure) to investigate the transcriptomic response of peripheral leukocytes in COPD is a step beyond the standard case-control transcriptomic profiling carried out so far, and has facilitated the identification of novel COPD and Smoking expression related signatures which differ in males and females.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2014, vol. 9, num. 5, p. e97491
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

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