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Title: | Evaluation of the effect of polymorphism on G-quadruplex-ligand interaction by means of spectroscopic and chromatographic techniques |
Author: | Benito, S. Ferrer, Assumpta Benabou Zdaou, Sanae Aviñó Andrés, Anna Eritja i Casadellà, Ramon Gargallo Gómez, Raimundo |
Keywords: | Polimorfisme (Cristal·lografia) Anàlisi multivariable Lligands G-estructures Polymorphism (Crystallography) Multivariate analysis Ligands G-structures |
Issue Date: | 6-Feb-2018 |
Publisher: | Elsevier B.V. |
Abstract: | Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose. The polymorphism of G-quadruplexes was characterized prior to the interaction studies. The ligand was shown to interact preferentially with the monomeric G-quadruplex; the binding stoichiometry was 1:1 and the binding constant was in the order of 105 M-1 for all three sequences. The results highlight the importance of DNA treatment prior to interaction studies |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.saa.2018.02.006 |
It is part of: | Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2018 |
URI: | http://hdl.handle.net/2445/119785 |
Related resource: | https://doi.org/10.1016/j.saa.2018.02.006 |
ISSN: | 1386-1425 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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