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Title: Invasive bacterial disease trends and characterization of group B streptococcal isolates among young infants in southern Mozambique, 2001-2015
Author: Sigauque, Betuel
Kobayashi, Miwako
Vubil, Delfino
Nhacolo, Ariel
Chauque, Alberto
Moaine, Benild
Massora, Sérgio
Mandomando, Inácio
Nhampossa, Tacilta
Bassat Orellana, Quique
Pimenta, Fabiana
Menéndez, Clara
Carvalho, Maria da Gloria
Macete, Eusébio
Schrag, Stephanie
Keywords: Malalties bacterianes
Bacterial diseases
Issue Date: 19-Jan-2018
Publisher: Public Library of Science (PLoS)
Abstract: BACKGROUND: Maternal group B streptococcal (GBS) vaccines under development hold promise to prevent GBS disease in young infants. Sub-Saharan Africa has the highest estimated disease burden, although data on incidence and circulating strains are limited. We described invasive bacterial disease (IBD) trends among infants <90 days in rural Mozambique during 2001-2015, with a focus on GBS epidemiology and strain characteristics. METHODS: Community-level birth and mortality data were obtained from Manhica's demographic surveillance system. IBD cases were captured through ongoing surveillance at Manhica district hospital. Stored GBS isolates from cases underwent serotyping by multiplex PCR, antimicrobial susceptibility testing, and whole genome sequencing. RESULTS: There were 437 IBD cases, including 57 GBS cases. Significant declines in overall IBD, neonatal mortality, and stillbirth rates were observed (P<0.0001), but not for GBS (P = 0.17). In 2015, GBS was the leading cause of young infant IBD (2.7 per 1,000 live births). Among 35 GBS isolates available for testing, 31 (88.6%) were highly related serotype III isolates within multilocus sequence types (STs) 17 (68.6%) or 109 (20.0%). All seven ST109 isolates (21.9%) had elevated minimum inhibitory concentration (MIC) to penicillin (>/=0.12 mug/mL) associated with penicillin-binding protein (PBP) 2x substitution G398A. Epidemiologic and molecular data suggest this is a well-established clone. CONCLUSION: A notable young infant GBS disease burden persisted despite improvements in overall maternal and neonatal health. We report an established strain with pbp2x point mutation, a first-step mutation associated with reduced penicillin susceptibility within a well-known virulent lineage in rural Mozambique. Our findings further underscores the need for non-antibiotic GBS prevention strategies.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2018, vol. 13, num. 1, p. e0191193
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (ISGlobal)

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