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DC Field | Value | Language |
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dc.contributor.author | Solé Ferré, Anna | - |
dc.contributor.author | Delagoutte, Emmanuelle | - |
dc.contributor.author | Ciudad i Gómez, Carlos Julián | - |
dc.contributor.author | Noé Mata, Verónica | - |
dc.contributor.author | Alberti, Patrizia | - |
dc.date.accessioned | 2018-03-02T14:59:52Z | - |
dc.date.available | 2018-03-02T14:59:52Z | - |
dc.date.issued | 2017-01-09 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/120404 | - |
dc.description.abstract | Polypurine reverse-Hoogsteen (PPRH) oligonucleotides are non-modified DNA molecules composed of two mirror-symmetrical polypurine stretches linked by a five-thymidine loop. They can fold into reverse-Hoogsteen hairpins and bind to their polypyrimidine target sequence by Watson-Crick bonds forming a three-stranded structure. They have been successfully used to knockdown gene expression and to repair single-point mutations in cells. In this work, we provide an in vitro characterization (UV and fluorescence spectroscopy, gel electrophoresis and nuclease assays) of the structure and stability of two repair-PPRH oligonucleotides and of the complexes they form with their single-stranded targets. We show that one PPRH oligonucleotide forms a hairpin, while the other folds, in potassium, into a guanine-quadruplex (G4). However, the hairpin-prone oligonucleotide does not form a triplex with its single-stranded target, while the G4-prone oligonucleotide converts from a G4 into a reverse-Hoogsteen hairpin forming a triplex with its target sequence. Our work proves, in particular, that folding of a PPRH oligonucleotide into a G4 does not necessarily impair sequence-specific DNA recognition by triplex formation. It also illustrates an original example of DNA structural conversion of a G4 into a reverse-Hoogsteen hairpin driven by triplex formation; this kind of conversion might occur at particular loci of genomic DNA. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/srep39898 | - |
dc.relation.ispartof | Scientific Reports, 2017, vol. 7 , num. 39898 | - |
dc.relation.uri | https://doi.org/10.1038/srep39898 | - |
dc.rights | cc-by (c) Solé, Anna et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | ADN | - |
dc.subject.classification | Estructura molecular | - |
dc.subject.other | DNA | - |
dc.subject.other | Molecular structure | - |
dc.title | Polypurine reverse-Hoogsteen (PPRH) oligonucleotides can form triplexes with their target sequences even under conditions where they fold into G-quadruplexes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 670332 | - |
dc.date.updated | 2018-03-02T14:59:52Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28067256 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
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File | Description | Size | Format | |
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670332.pdf | 1.38 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License