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DC Field | Value | Language |
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dc.contributor.author | Mick, Virginie | - |
dc.contributor.author | Domínguez Luzón, Ma. Ángeles (María Ángeles) | - |
dc.contributor.author | Tubau, Fe | - |
dc.contributor.author | Liñares Louzao, Josefina | - |
dc.contributor.author | Pujol Rojo, Miquel | - |
dc.contributor.author | Martín, Rogelio | - |
dc.date.accessioned | 2018-03-13T09:22:56Z | - |
dc.date.available | 2018-03-13T09:22:56Z | - |
dc.date.issued | 2010-03-04 | - |
dc.identifier.issn | 1471-2180 | - |
dc.identifier.uri | http://hdl.handle.net/2445/120647 | - |
dc.description.abstract | Background: Methicillin-resistant S. aureus (MRSA) has been endemic in Hospital Universitari de Bellvitge, Barcelona, since 1990. During the 1990-95 period the Iberian clone (ST-247; SCCmec-I) was dominant. Isolates of clonal complex 5 (ST-125; SCCmec-IV) gradually replaced the Iberian clone from 1996 to 2003. A new multiresistant MRSA phenotype showing rifampicin resistance emerged in 2004 and rapidly increased from 25% in 2004 to 45% in 2006. The aims of this study were i) the molecular characterisation of rifampicin resistant MRSA isolates, ii) the study of the rifampicin resistance expression by disk diffusion, microdilution and E-test, and iii) the analysis of the rpoB gene mutations involved in rifampicin resistance. Results: A sample of representative 108 rifampicin-resistant MRSA isolates belonged to a single PFGE genotype, ST-228, SCCmec type I and spa type t041. Of 108 isolates, 104 (96%) had a low-level rifampicin resistance (MICs, 2 to 4 mg/L) and 4 a high-level rifampicin resistance (MICs, 128 - ≥ 256 mg/L). Disk diffusion and E-test methods failed to identify a low-level rifampicin resistance in 20 and 12 isolates, respectively. A low-level rifampicin resistance was associated with amino acid substitution 481His/Asn in the beta-subunit of RNA polymerase. Isolates with a high-level rifampicin resistance carried additional mutations in the rpoB gene. Conclusions: The emergence of MRSA clone ST228-SCCmec I, related to the Southern Germany clone, involved a therapeutical challenge for treating serious MRSA infections. Decreased susceptibility to rifampicin in MRSA strains of ST228-SCCmecI was associated with one or two specific mutations in the rpoB gene. One fifth of isolates with low-level rifampicin-resistance were missed by the diffusion methods. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/1471-2180-10-68 | - |
dc.relation.ispartof | BMC Microbiology, 2010, vol. 10, p. 68 | - |
dc.relation.uri | https://doi.org/10.1186/1471-2180-10-68 | - |
dc.rights | cc-by (c) Mick, Virginie et al., 2010 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Rifampicina | - |
dc.subject.classification | Espanya | - |
dc.subject.classification | Resistència als medicaments | - |
dc.subject.classification | Hospital de Bellvitge Prínceps d'Espanya | - |
dc.subject.other | Rifampin | - |
dc.subject.other | Spain | - |
dc.subject.other | Drug resistance | - |
dc.title | Molecular characterization of resistance to Rifampicin in an emerging hospital-associated Methicillin-resistant Staphylococcus aureus clone ST228, Spain | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 615141 | - |
dc.date.updated | 2018-03-13T09:22:56Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 20202188 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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615141.pdf | 644.56 kB | Adobe PDF | View/Open |
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