Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/120709
Title: | AFM and Microrheology in the Zebrafish Embryo Yolk Cell |
Author: | Marsal, Maria Jorba, Ignasi Rebollo, Elena Luque González, Tomás Navajas Navarro, Daniel Martín Blanco, Enrique |
Keywords: | Peix zebra Models animals en la investigació Reologia (Biologia) Zebra danio Animal models in research Rheology (Biology) |
Issue Date: | 29-Nov-2017 |
Abstract: | Elucidating the factors that direct the spatio-temporal organization of evolving tissues is one of the primary purposes in the study of development. Various propositions claim to have been important contributions to the understanding of the mechanical properties of cells and tissues in their spatiotemporal organization in different developmental and morphogenetic processes. However, due to the lack of reliable and accessible tools to measure material properties and tensional parameters in vivo, validating these hypotheses has been difficult. Here we present methods employing atomic force microscopy (AFM) and particle tracking with the aim of quantifying the mechanical properties of the intact zebrafish embryo yolk cell during epiboly. Epiboly is an early conserved developmental process whose study is facilitated by the transparency of the embryo. These methods are simple to implement, reliable, and widely applicable since they overcome intrusive interventions that could affect tissue mechanics. A simple strategy was applied for the mounting of specimens, AFM recording, and nanoparticle injections and tracking. This approach makes these methods easily adaptable to other developmental times or organisms. |
Note: | Reproducció del document publicat a: https://doi.org/10.3791/56224 |
It is part of: | Jove-Journal Of Visualized Experiments, 2017, num. 129, p. e56224 |
URI: | http://hdl.handle.net/2445/120709 |
Related resource: | https://doi.org/10.3791/56224 |
ISSN: | 1940-087X |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
678636.pdf | 539.97 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.