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http://hdl.handle.net/2445/120715
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DC Field | Value | Language |
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dc.contributor.author | Valon, Léo | - |
dc.contributor.author | Marín Llauradó, Ariadna | - |
dc.contributor.author | Wyatt, Thomas | - |
dc.contributor.author | Charras, Guillaume | - |
dc.contributor.author | Trepat Guixer, Xavier | - |
dc.date.accessioned | 2018-03-14T13:59:23Z | - |
dc.date.available | 2018-03-14T13:59:23Z | - |
dc.date.issued | 2017-02-10 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/2445/120715 | - |
dc.description.abstract | Contractile forces are the end effectors of cell migration, division, morphogenesis, wound healing and cancer invasion. Here we report optogenetic tools to upregulate and downregulate such forces with high spatiotemporal accuracy. The technology relies on controlling the subcellular activation of RhoA using the CRY2/CIBN light-gated dimerizer system. We fused the catalytic domain (DHPH domain) of the RhoA activator ARHGEF11 to CRY2-mCherry (optoGEF-RhoA) and engineered its binding partner CIBN to bind either to the plasma membrane or to the mitochondrial membrane. Translocation of optoGEF-RhoA to the plasma membrane causes a rapid and local increase in cellular traction, intercellular tension and tissue compaction. By contrast, translocation of optoGEF-RhoA to mitochondria results in opposite changes in these physical properties. Cellular changes in contractility are paralleled by modifications in the nuclear localization of the transcriptional regulator YAP, thus showing the ability of our approach to control mechanotransductory signalling pathways in time and space. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/ncomms14396 | - |
dc.relation.ispartof | Nature Communications, 2017, vol. 8, num. 14396 | - |
dc.relation.uri | https://doi.org/10.1038/ncomms14396 | - |
dc.rights | cc-by (c) Valon, Léo et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Biofísica | - |
dc.subject.classification | Citosquelet | - |
dc.subject.other | Biophysics | - |
dc.subject.other | Cytoskeleton | - |
dc.title | Optogenetic control of cellular forces and mechanotransduction | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 670887 | - |
dc.date.updated | 2018-03-14T13:59:23Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/647186/EU//MolCellTissMech | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28186127 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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670887.pdf | 4.56 MB | Adobe PDF | View/Open |
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