Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/121074
Title: Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk
Author: Rubio, Mercedes
Bustamante Pineda, Mariona
Hernández Ferrer, Carles
Fernández Orth, Dietmar
Pantano, Lorena
Sarria, Yaris
Piqué Borras, Maria
Vellve, Kilian
Agramunt, Silvia
Carreras, Ramon
Estivill, Xavier, 1955-
González, Juan Ramón
Mayor Aparicio, Alfredo Gabriel
Keywords: Micro RNAs
Llet materna
MicroRNAs
Breast milk
Issue Date: 5-Mar-2018
Publisher: Public Library of Science (PLoS)
Abstract: Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk samples from 15 healthy postpartum mothers. Small RNA libraries were prepared with the NEBNext(R) small RNA library preparation kit and sequenced in an Illumina HiSeq2000 platform. miRNAs, isomiRs and clusters of small RNAs were annotated using seqBuster/seqCluster framework in 5 plasma and 10 milk samples that passed the initial quality control. The RNA yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL (SD: 3767) for plasma and breast milk, respectively. Mean number of good quality reads was 4.04 million (M) (40.01% of the reads) in plasma and 12.5M (89.6%) in breast milk. One thousand one hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA clusters that included piwi-interfering RNAs (piRNAs), tRNAs, small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs) were detected. Samples grouped by biofluid, with 308 miRNAs, 1,790 isomiRs and 778 small RNA clusters differentially detected. In summary, plasma and milk showed a different small RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs were identified, confirming the presence of non-miRNA species in plasma, and describing them for the first time in milk.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0193527
It is part of: PLoS One, 2018, vol. 13, num. 3, p. e0193527
URI: http://hdl.handle.net/2445/121074
Related resource: http://dx.doi.org/10.1371/journal.pone.0193527
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (ISGlobal)

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