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http://hdl.handle.net/2445/121818
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DC Field | Value | Language |
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dc.contributor.author | Jayaraman, Anusha | - |
dc.contributor.author | Kumar, Praveen | - |
dc.contributor.author | Marín Martínez, Silvia | - |
dc.contributor.author | Atauri Carulla, Ramón de | - |
dc.contributor.author | Mateo González, Francesca | - |
dc.contributor.author | Thomson, Timothy M. | - |
dc.contributor.author | Centelles Serra, Josep Joan | - |
dc.contributor.author | Graham, Stewart F. | - |
dc.contributor.author | Cascante i Serratosa, Marta | - |
dc.date.accessioned | 2018-04-24T11:34:01Z | - |
dc.date.available | 2018-04-24T11:34:01Z | - |
dc.date.issued | 2018-02-21 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/121818 | - |
dc.description.abstract | Tumour angiogenesis is an important hallmark of cancer and the study of its metabolic adaptations, downstream to any cellular change, can reveal attractive targets for inhibiting cancer growth. In the tumour microenvironment, endothelial cells (ECs) interact with heterogeneous tumour cell types that drive angiogenesis and metastasis. In this study we aim to characterize the metabolic alterations in ECs influenced by the presence of tumour cells with extreme metastatic abilities. Human umbilical vein endothelial cells (HUVECs) were subjected to different microenvironmental conditions, such as the presence of highly metastatic PC-3M and highly invasive PC-3S prostate cancer cell lines, in addition to the angiogenic activator vascular endothelial growth factor (VEGF), under normoxia. Untargeted high resolution liquid chromatography-mass spectrometry (LC-MS) based metabolomics revealed significant metabolite differences among the various conditions and a total of 25 significantly altered metabolites were identified including acetyl L-carnitine, NAD+, hypoxanthine, guanine and oleamide, with profile changes unique to each of the experimental conditions. Biochemical pathway analysis revealed the importance of fatty acid oxidation and nucleotide salvage pathways. These results provide a global metabolic preview that could help in selectively targeting the ECs aiding in either cancer cell invasion or metastasis in the heterogeneous tumour microenvironment. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0192175 | - |
dc.relation.ispartof | PLoS One, 2018, vol. 13, num. 2, p. e0192175 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0192175 | - |
dc.rights | cc-by (c) Jayaraman, Anusha et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Biologia molecular | - |
dc.subject.classification | Interacció cel·lular | - |
dc.subject.classification | Càncer | - |
dc.subject.other | Molecular biology | - |
dc.subject.other | Cell interaction | - |
dc.subject.other | Cancer | - |
dc.title | Untargeted metabolomics reveals distinct metabolic reprogramming in endothelial cells co-cultured with CSC and non-CSC prostate cancer cell subpopulations. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 679075 | - |
dc.date.updated | 2018-04-24T11:34:02Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/264780/EU//METAFLUX | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29466368 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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679075.pdf | 3.39 MB | Adobe PDF | View/Open |
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