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Title: An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
Author: Orengo Ferriz, Dorcas J.
Puerma Rodríguez, Eva María
Cereijo, Unai
Salguero, David
Aguadé Porres, Montserrat
Keywords: Drosòfila
Genètica evolutiva
Evolutionary genetics
Issue Date: 5-Oct-2017
Publisher: Nature Publishing Group
Abstract: Inversion polymorphism is widespread in the Drosophila genus as well as in other dipteran genera. The presence of polytene chromosomes in some insect organs and, thus, the possibility to observe the different arrangements generated by inversions through a microscope enhanced the cytological study of this structural polymorphism. In several Drosophila species, these studies provided evidence for the adaptive character of this polymorphism, which together with the standing interest to uncover targets of natural selection has led to a renewed interest for inversion polymorphism. Our recent molecular characterization of the breakpoint regions of five inversions of the E chromosome of D. subobscura has allowed us to design a PCR-based strategy to molecularly identify the different chromosomal arrangements and, most importantly, to determine the E chromosome karyotype of medium- and large-sized samples from natural populations. Individuals of a test sample that were both cytologically and molecularly karyotyped were used to establish the strategy that was subsequently applied to karyotype a larger sample. Our strategy has proved to be robust and time efficient, and it lays therefore the groundwork for future studies of the E chromosome structural polymorphism through space and time, and of its putative contribution to adaptation.
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It is part of: Scientific Reports, 2017, vol. 7, num. 12717
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Institut de Recerca de la Biodiversitat (IRBio))

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