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Title: Metabotropic glutamate 1α and adenosine A1 receptors assemble into functionally interacting complexes
Author: Ciruela Alférez, Francisco
Escriche, Marisol
Burgueño, Javier
Angulo, Ester
Casadó, Vicent
Soloviev, Mikhail M.
Canela Campos, Enric I.
Mallol Montero, Josefa
Chan, Wai-Yee
Lluís i Biset, Carme
McIlhinney, R. A. Jeffrey
Franco Fernández, Rafael
Keywords: Adenosina
Àcid glutàmic
Neural transmission
Glutamic acid
Issue Date: May-2001
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Recently, evidence has emerged that seven transmembrane G protein-coupled receptors may be present as homo- and heteromers in the plasma membrane. Here we describe a new molecular and functional interaction between two functionally unrelated types of G protein-coupled receptors, namely the metabotropic glutamate type 1alpha (mGlu(1alpha) receptor) and the adenosine A1 receptors in cerebellum, primary cortical neurons, and heterologous transfected cells. Co-immunoprecipitation experiments showed a close and subtype-specific interaction between mGlu(1alpha) and A1 receptors in both rat cerebellar synaptosomes and co-transfected HEK-293 cells. By using transiently transfected HEK-293 cells a synergy between mGlu(1alpha) and A1 receptors in receptor-evoked [Ca(2+)](i) signaling has been shown. In primary cultures of cortical neurons we observed a high degree of co-localization of the two receptors, and excitotoxicity experiments in these cultures also indicate that mGlu(1alpha) and A1 receptors are functionally related. Our results provide a molecular basis for adenosine/glutamate receptors cross-talk and open new perspectives for the development of novel agents to treat neuropsychiatric disorders in which abnormal glutamatergic neurotransmission is involved.
Note: Reproducció del document publicat a:
It is part of: Journal of Biological Chemistry, 2001, vol. 276, num. 21, p. 18345-18351
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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