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Title: Coaggregation, cointernalization, and codesensitization of adenosine A2A receptors and dopamine D2 receptors
Author: Hillion, Joëlle
Canals, Meritxell
Torvinen, Maria
Casadó, Vicent
Scott, Rizaldy
Terasmaa, Anton
Hansson, Anita
Watson, Stanley
Olah, Mark E.
Mallol Montero, Josefa
Canela Campos, Enric I.
Zoli, Michele
Agnati, Luigi F.
Ibanez, Carlos F.
Lluís i Biset, Carme
Franco Fernández, Rafael
Ferré, Sergi
Fuxe, Kjell
Keywords: Receptors cel·lulars
Malalties del sistema nerviós
Cell receptors
Nervous system diseases
Issue Date: May-2002
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A(2A) receptors (A(2A)R) and dopamine D(2) receptors (D(2)R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D(2)R and in cultured striatal cells. A(2A)R/D(2)R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D(2)R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D(2)R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D(2)R agonists in D(2)R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D(2)R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D(2)R are involved.
Note: Reproducció del document publicat a:
It is part of: Journal of Biological Chemistry, 2002, vol. 277, num. 20, p. 18091-18097
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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