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|Title:||Actin-binding protein α-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor|
Mallol Montero, Josefa
Canela Campos, Enric I.
Robbins, Melanie J.
Lluís i Biset, Carme
Franco Fernández, Rafael
McIlhinney, R. A. Jeffrey
Ciruela Alférez, Francisco
|Publisher:||American Society for Biochemistry and Molecular Biology|
|Abstract:||Receptors for neurotransmitters require scaffolding proteins for membrane microdomain targeting and for regulating receptor function. Using a yeast two-hybrid screen, α-actinin-1, a major F-actin cross-linking protein, was identified as a binding partner for the C-terminal domain of metabotropic glutamate receptor type 5b (mGlu5b receptor). Co-expression, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between mGlu5b receptor and α-actinin-1 in both transfected HEK-293 cells and rat striatum. The interaction of α-actinin-1 with mGlu5b receptor modulated the cell surface expression of the receptor. This was dependent on the binding of α-actinin-1 to the actin cytoskeleton. In addition, the α-actinin-1/mGlu5b receptor interaction regulated receptor-mediated activation of the mitogen-activated protein kinase pathway. Together, these findings indicate that there is an α-actinin-1-dependent mGlu5b receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1074/jbc.M608880200|
|It is part of:||Journal of Biological Chemistry, 2007, vol. 282, num. 16, p. 12143-12153|
|Appears in Collections:||Articles publicats en revistes (Bioquímica i Biomedicina Molecular)|
Articles publicats en revistes (Patologia i Terapèutica Experimental)
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