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Title: | Cell culture replication of a genotype 1b hepatitis C virus isolate cloned from a patient who underwent liver transplantation |
Author: | Koutsoudakis, George Pérez del Pulgar Gallart, Sofía Coto Llerena, Mairene González, Patricia Dragun, Jakub Mensa Garrigosa, Laura Crespo, Gonzalo Navasa, Miquel Forns, Xavier |
Keywords: | Virus de l'hepatitis C Cultiu cel·lular Malalties del fetge Hepatitis C virus Cell culture Liver diseases |
Issue Date: | 24-Aug-2011 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | The introduction of the genotype 2a isolate JFH1 was a major breakthrough in the field of hepatitis C virus (HCV), allowing researchers to study the complete life cycle of the virus in cell culture. However, fully competent culture systems encompassing the most therapeutically relevant HCV genotypes are still lacking, especially for the highly drug-resistant genotype 1b. For most isolated HCV clones, efficient replication in cultured hepatoma cells requires the introduction of replication-enhancing mutations. However, such mutations may interfere with viral assembly, as occurs in the case of the genotype 1b isolate Con1. In this study, we show that a clinical serum carrying a genotype 1b virus with an exceptionally high viral load was able to infect Huh7.5 cells. Similar to previous reports, inoculation of Huh7.5 cells by natural virus is very inefficient compared to infection by cell culture HCV. A consensus sequence of a new genotype 1b HCV isolate was cloned from the clinical serum (designated Barcelona HCV1), and then subjected to replication studies. This virus replicated poorly in a transient fashion in Huh7.5 cells after electroporation with in vitro transcribed RNA. Nonetheless, approximately 3 weeks post electroporation and thereafter, core protein-positive cells were detected by immunofluorescence. Surprisingly, small amounts of core protein were also measurable in the supernatant of electroporated cells, suggesting that HCV particles might be assembled and released. Our findings not only enhance the current method of cloning in vitro HCV replication-competent isolates, but also offer valuable insights for the realization of fully competent culture systems for HCV. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0023587 |
It is part of: | PLoS One, 2011, vol. 6, num. 8, p. e23587 |
URI: | http://hdl.handle.net/2445/122863 |
Related resource: | https://doi.org/10.1371/journal.pone.0023587 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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