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http://hdl.handle.net/2445/123190
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DC Field | Value | Language |
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dc.contributor.author | Peró Gascón, Roger | - |
dc.contributor.author | Sanz Nebot, María Victoria | - |
dc.contributor.author | Berezovski, Maxim V. | - |
dc.contributor.author | Benavente Moreno, Fernando J. (Julián) | - |
dc.date.accessioned | 2018-06-21T15:03:28Z | - |
dc.date.available | 2019-05-08T05:10:13Z | - |
dc.date.issued | 2018-05-07 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/2445/123190 | - |
dc.description.abstract | In this paper, an on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) method is described for the purification, preconcentration, separation, and characterization of endogenous microRNA (miRNA) and their post-transcriptional modifications in serum. First, analysis by CE-MS was optimized using a standard mixture of hsa-miR-21-5p (miR-21-5p) and hsa-let-7g-5p (let-7g-5p). For SPE-CE-MS, a commercial silicon carbide (SiC) resin was used to prepare the microcartridges. Under the optimized conditions with standards, the microcartridge lifetime (>25 analyses) and repeatability (2.8% RSD for the migration times; 4.4 and 6.4% RSD for the miR-21-5p and let-7g-5p peak areas, respectively) were good, the method was linear between 25 and 100 nmol·L-1, and the limit of detection (LOD) was around 10 nmol·L-1 (50 times lower than by CE-MS). In order to analyze human serum samples, an off-line sample pretreatment based on phenol/chloroform/isoamyl alcohol (PCA) extraction was necessary prior to SPE-CE-MS. The potential of the SPE-CE-MS method to screen for B-cell chronic lymphocytic leukemia (CLL) was demonstrated by an analysis of serum samples from healthy controls and patients. MicroRNAs, specifically miR-21-5p and a 23 nucleotide long 5'-phosphorylated miRNA with 3'-uridylation (iso-miR-16-5p), were only detected in the CLL patients. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1021/acs.analchem.8b00405 | - |
dc.relation.ispartof | Analytical Chemistry, 2018, vol. 90, p. 6618-6625 | - |
dc.relation.uri | https://doi.org/10.1021/acs.analchem.8b00405 | - |
dc.rights | (c) American Chemical Society , 2018 | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.other | Capillary electrophoresis | - |
dc.subject.other | Mass spectrometry | - |
dc.subject.other | MicroRNAs | - |
dc.title | Analysis of circulating microRNAs and their post-transcriptional modifications in cancer serum by on-line solid-phase extraction-capillary electrophoresis-mass spectrometry | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 680822 | - |
dc.date.updated | 2018-06-21T15:03:28Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29730931 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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680822.pdf | 1.17 MB | Adobe PDF | View/Open |
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