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Title: | Rare variants in calcium homeostasis modulator 1 (CALHM1) found in early onset Alzheimer's disease patients alter calcium homeostasis. |
Author: | Rubio Moscardo, Fanny Setó Salvia, Núria Pera, Marta Bosch Morató, Mònica Plata, Cristina Belbin, Olivia Gené, Gemma Dols Icardo, Oriol Ingelsson, Martin Helisalmi, Seppo Soininen, Hilkka Hiltunen, Mikko Giedraitis, Vilmantas Lannfelt, Lars Frank García, Ana Bullido, María Jesús Combarros, Onofre Sánchez Juan, Pascual Boada, Mercè Tárraga, Lluís Pastor, Pau Pérez Tur, Jordi Baquero, Miquel Molinuevo, José Luis Sánchez del Valle Díaz, Raquel Fuentes Prior, Pablo Fortea Ormaechea, Juan Blesa González, Rafael Muñoz, Francisco J. Lleó Bisa, Alberto Valverde, Miguel Ángel Clarimón, Jordi |
Keywords: | Malaltia d'Alzheimer Homeòstasi Calci Alzheimer's disease Homeostasis Calcium |
Issue Date: | 17-Sep-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Calcium signaling in the brain is fundamental to the learning and memory process and there is evidence to suggest that its dysfunction is involved in the pathological pathways underlying Alzheimer's disease (AD). Recently, the calcium hypothesis of AD has received support with the identification of the non-selective Ca2+-permeable channel CALHM1. A genetic polymorphism (p. P86L) in CALHM1 reduces plasma membrane Ca2+ permeability and is associated with an earlier age-at-onset of AD. To investigate the role of CALHM1 variants in early-onset AD (EOAD), we sequenced all CALHM1 coding regions in three independent series comprising 284 EOAD patients and 326 controls. Two missense mutations in patients (p.G330D and p.R154H) and one (p.A213T) in a control individual were identified. Calcium imaging analyses revealed that while the mutation found in a control (p.A213T) behaved as wild-type CALHM1 (CALHM1-WT), a complete abolishment of the Ca2+ influx was associated with the mutations found in EOAD patients (p.G330D and p.R154H). Notably, the previously reported p. P86L mutation was associated with an intermediate Ca2+ influx between the CALHM1-WT and the p.G330D and p.R154H mutations. Since neither expression of wild-type nor mutant CALHM1 affected amyloid ß-peptide (Aß) production or Aß-mediated cellular toxicity, we conclude that rare genetic variants in CALHM1 lead to Ca2+ dysregulation and may contribute to the risk of EOAD through a mechanism independent from the classical Aß cascade |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0074203 |
It is part of: | PLoS One, 2013, vol. 8, num. 9, p. 1-8 |
URI: | http://hdl.handle.net/2445/123830 |
Related resource: | https://doi.org/10.1371/journal.pone.0074203 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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