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http://hdl.handle.net/2445/123874
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DC Field | Value | Language |
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dc.contributor.author | Höftberger, Romana | - |
dc.contributor.author | Sabater, Lidia | - |
dc.contributor.author | Marignier, Romain | - |
dc.contributor.author | Aboul-Enein, Fahmy | - |
dc.contributor.author | Bernard-Valnet, Raphaël | - |
dc.contributor.author | Rauschka, Helmut | - |
dc.contributor.author | Ruiz, Anne | - |
dc.contributor.author | Blanco Morgado, Yolanda | - |
dc.contributor.author | Graus Ribas, Francesc | - |
dc.contributor.author | Dalmau Obrador, Josep | - |
dc.contributor.author | Saiz Hinarejos, Albert | - |
dc.date.accessioned | 2018-07-24T12:57:29Z | - |
dc.date.available | 2018-07-24T12:57:29Z | - |
dc.date.issued | 2013-11-04 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/123874 | - |
dc.description.abstract | Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0079083 | - |
dc.relation.ispartof | PLoS One, 2013, vol. 8, num. 11, p. 1-6 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0079083 | - |
dc.rights | cc-by (c) Höftberger, Romana et al., 2013 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Immunohistoquímica | - |
dc.subject.classification | Immunoglobulines | - |
dc.subject.other | Immunohistochemistry | - |
dc.subject.other | Immunoglobulins | - |
dc.title | An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 654344 | - |
dc.date.updated | 2018-07-24T12:57:29Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24223884 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Medicina) |
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654344.pdf | 787.77 kB | Adobe PDF | View/Open |
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