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http://hdl.handle.net/2445/124235
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DC Field | Value | Language |
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dc.contributor.author | Barrio Tofiño, Ester | - |
dc.contributor.author | López Causapé, Carla | - |
dc.contributor.author | Cabot, Gabriel | - |
dc.contributor.author | Rivera, Alba | - |
dc.contributor.author | Benito Hernández, M. Natividad de | - |
dc.contributor.author | Segura, Concepción | - |
dc.contributor.author | Montero, María Milagro | - |
dc.contributor.author | Sorli, Luisa | - |
dc.contributor.author | Tubau, Fe | - |
dc.contributor.author | Gómez-Zorrilla Martín, Silvia | - |
dc.contributor.author | Tormo, Nuria | - |
dc.contributor.author | Durá Navarro, Raquel | - |
dc.contributor.author | Viedma, Esther | - |
dc.contributor.author | Resino Foz, Elena | - |
dc.contributor.author | Fernández Martínez, Marta | - |
dc.contributor.author | González Rico, Claudia | - |
dc.contributor.author | Alejo-Cancho, Izaskun | - |
dc.contributor.author | Martínez, José Antonio (Martínez Martínez) | - |
dc.contributor.author | Labayru Echverria, Cristina | - |
dc.contributor.author | Dueñas, Carlos J. | - |
dc.contributor.author | Ayestaran, Ignacio | - |
dc.contributor.author | Zamorano, Laura | - |
dc.contributor.author | Martínez Martínez, Luis | - |
dc.contributor.author | Horcajada Gallego, Juan Pablo | - |
dc.contributor.author | Oliver, Antonio | - |
dc.date.accessioned | 2018-09-03T14:13:06Z | - |
dc.date.available | 2018-09-03T14:13:06Z | - |
dc.date.issued | 2017-11-01 | - |
dc.identifier.uri | http://hdl.handle.net/2445/124235 | - |
dc.description.abstract | This study assessed the molecular epidemiology, resistance mechanisms, and susceptibility profiles of a collection of 150 extensively drug-resistant (XDR) Pseudomonas aeruginosa clinical isolates obtained from a 2015 Spanish multicenter study, with a particular focus on resistome analysis in relation to ceftolozane-tazobactam susceptibility. Broth microdilution MICs revealed that nearly all (> 95%) of the isolates were nonsusceptible to piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, and ciprofloxacin. Most of them were also resistant to tobramycin (77%), whereas nonsusceptibility rates were lower for ceftolozane-tazobactam (31%), amikacin (7%), and colistin (2%). Pulsed-field gel electrophoresis-multilocus sequence typing (PFGE-MLST) analysis revealed that nearly all of the isolates belonged to previously described high-risk clones. Sequence type 175 (ST175) was detected in all 9 participating hospitals and accounted for 68% (n = 101) of the XDR isolates, distantly followed by ST244 (n = 16), ST253 (n = 12), ST235 (n = 8), and ST111 (n = 2), which were detected only in 1 to 2 hospitals. Through phenotypic and molecular methods, the presence of horizontally acquired carbapenemases was detected in 21% of the isolates, mostly VIM (17%) and GES enzymes (4%). At least two representative isolates from each clone and hospital (n = 44) were fully sequenced on an illumina MiSeq. Classical mutational mechanisms, such as those leading to the overexpression of the beta-lactamase AmpC or efflux pumps, OprD inactivation, and/or quinolone resistance-determining regions (QRDR) mutations, were confirmed in most isolates and correlated well with the resistance phenotypes in the absence of horizontally acquired determinants. Ceftolozane-tazobactam resistance was not detected in carbapenemase-negative isolates, in agreement with sequencing data showing the absence of ampC mutations. The unique set of mutations responsible for the XDR phenotype of ST175 clone documented 7 years earlier were found to be conserved, denoting the long-term persistence of this specific XDR lineage in Spanish hospitals. Finally, other potentially relevant mutations were evidenced, including those in penicillin-binding protein 3 (PBP3), which is involved in beta-lactam (including ceftolozane-tazobactam) resistance, and FusA1, which is linked to aminoglycoside resistance. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1128/AAC.01589-17 | - |
dc.relation.ispartof | Antimicrobial Agents And Chemotherapy, 2017, vol. 61, num. 11 | - |
dc.relation.uri | https://doi.org/10.1128/AAC.01589-17 | - |
dc.rights | (c) American Society for Microbiology, 2017 | - |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Epidemiologia molecular | - |
dc.subject.classification | Pseudomonas | - |
dc.subject.other | Molecular epidemiology | - |
dc.title | Genomics And Susceptibility Profiles Of Extensively Drug-resistant Pseudomonas Aeruginosa Isolates From Spain | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2018-07-24T11:57:17Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/952491/EU//AmReSu | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28874376 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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del Barrio-TofinoE.pdf | 1.38 MB | Adobe PDF | View/Open |
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