Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/124629
Title: Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes
Author: Madeira, Ana
Camps Camprubí, Marta
Zorzano Olarte, Antonio
Moura, Teresa F.
Soveral, Graça
Keywords: Cèl·lules animals
Glicerina
Teixit adipós
Animal cells
Glycerin
Adipose tissues
Issue Date: 20-Dec-2013
Publisher: Public Library of Science (PLoS)
Abstract: The plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role in glycerol release/uptake in adipocytes has been postulated and correlated with obesity onset. However, some studies have contradicted this view. Based on this situation, we have re-assessed the precise localization of AQP7 in adipose tissue and analyzed its function as a water and/or glycerol channel in adipose cells. Fractionation of mice adipose tissue revealed that AQP7 is located in both adipose and stromal vascular fractions. Moreover, AQP7 was the only aquaglyceroporin expressed in adipose tissue and in 3T3-L1 adipocytes. By overexpressing the human AQP7 in 3T3-L1 adipocytes it was possible to ascertain its role as a water and glycerol channel in a gain-of-function scenario. AQP7 expression had no effect in equilibrium cell volume but AQP7 loss of function correlated with higher triglyceride content. Furthermore it is also reported for the first time a negative correlation between water permeability and the cell non-osmotic volume supporting the observation that AQP7 depleted cells are more prone to lipid accumulation. Additionally, the strong positive correlation between the rates of water and glycerol transport highlights the role of AQP7 as both a water and a glycerol channel and reflects its expression levels in cells. In all, our results clearly document a direct involvement of AQP7 in water and glycerol transport, as well as in triglyceride content in adipocytes.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0083442
It is part of: PLoS One, 2013, vol. 8, num. 12, p. 1-10
URI: http://hdl.handle.net/2445/124629
Related resource: https://doi.org/10.1371/journal.pone.0083442
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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