Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/124812
Title: Evaluation of alpha 1-antitrypsin and the levels of mRNA expression of matrix metalloproteinase 7, urokinase type plasminogen activator receptor and COX-2 for the diagnosis of colorectal cancer
Author: Bujanda, Luis
Sarasqueta, Cristina
Cosme, Angel
Hijona, Elizabeth
Enríquez Navascués, José M.
Placer, Carlos
Villarreal, Eloisa
Herreros Villanueva, Marta
Giraldez, María D.
Gironella, Meritxell
Balaguer Prunés, Francesc
Castells Garangou, Antoni
Keywords: Càncer colorectal
Càncer
Expressió gènica
Colorectal cancer
Cancer
Gene expression
Issue Date: 2-Jan-2013
Publisher: Public Library of Science (PLoS)
Abstract: Background Colorectal cancer (CRC) is the second most common cause of death from cancer in both men and women in the majority of developed countries. Molecular tests of blood could potentially provide this ideal screening tool. Aim Our objective was to assess the usefulness of serum markers and mRNA expression levels in the diagnosis of CRC. Methods In a prospective study, we measured mRNA expression levels of 13 markers (carbonic anhydrase, guanylyl cyclase C, plasminogen activator inhibitor, matrix metalloproteinase 7 (MMP7), urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator, survivin, tetranectin, vascular endothelial growth factor (VEGF), cytokeratin 20, thymidylate synthase, cyclooxygenase 2 (COX-2), and CD44) and three proteins in serum (alpha 1 antitrypsin, carcinoembryonic antigen (CEA) and activated C3 in 42 patients with CRC and 33 with normal colonoscopy results. Results Alpha 1-antitrypsin was the serum marker that was most useful for CRC diagnosis (1.79±0.25 in the CRC group vs 1.27±0.25 in the control group, P<0.0005). The area under the ROC curve for alpha 1-antitrypsin was 0.88 (0.79-0.96). The mRNA expression levels of five markers were statistically different between CRC cases and controls: those for which the ROC area was over 75% were MMP7 (0.81) and tetranectin (0.80), COX-2 (0.78), uPAR (0.78) and carbonic anhydrase (0.77). The markers which identified early stage CRC (Stages I and II) were alpha 1-antitrypsin, uPAR, COX-2 and MMP7. Conclusions Serum alpha 1-antitrypsin and the levels of mRNA expression of MMP7, COX-2 and uPAR have good diagnostic accuracy for CRC, even in the early stages.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0051810
It is part of: PLoS One, 2013, vol. 8, num. 1, p. e51810
URI: http://hdl.handle.net/2445/124812
Related resource: https://doi.org/10.1371/journal.pone.0051810
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

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