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http://hdl.handle.net/2445/124984
Title: | Loss of Symhc1 or Hsp90, function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos |
Author: | Codina, Marta Gutiérrez Fruitós, Joaquín Kao, Joseph P. Y. Du, Shao Jun Li, Junling |
Keywords: | Peix zebra Contracció muscular Proteïnes Zebra danio Muscle contraction Proteins |
Issue Date: | 1-Jan-2010 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Background Myofibrillogenesis requires the correct folding and assembly of sarcomeric proteins into highly organized sarcomeres. Heat shock protein 90α1 (Hsp90α1) has been implicated as a myosin chaperone that plays a key role in myofibrillogenesis. Knockdown or mutation of hsp90α1 resulted in complete disorganization of thick and thin filaments and M- and Z-line structures. It is not clear whether the disorganization of these sarcomeric structures is due to a direct effect from loss of Hsp90α1 function or indirectly through the disorganization of myosin thick filaments. Methodology/Principal Findings In this study, we carried out a loss-of-function analysis of myosin thick filaments via gene-specific knockdown or using a myosin ATPase inhibitor BTS (N-benzyl-p-toluene sulphonamide) in zebrafish embryos. We demonstrated that knockdown of myosin heavy chain 1 (myhc1) resulted in sarcomeric defects in the thick and thin filaments and defective alignment of Z-lines. Similarly, treating zebrafish embryos with BTS disrupted thick and thin filament organization, with little effect on the M- and Z-lines. In contrast, loss of Hsp90α1 function completely disrupted all sarcomeric structures including both thick and thin filaments as well as the M- and Z-lines. Conclusion/Significance Together, these studies indicate that the hsp90α1 mutant phenotype is not simply due to disruption of myosin folding and assembly, suggesting that Hsp90α1 may play a role in the assembly and organization of other sarcomeric structures. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0008416 |
It is part of: | PLoS One, 2010, vol. 5, num. 1, p. 1-9 |
URI: | http://hdl.handle.net/2445/124984 |
Related resource: | https://doi.org/10.1371/journal.pone.0008416 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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