Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/125364
Title: Regulation of human cerebrospinal fluid malate dehydrogenase 1 in sporadic Creutzfeldt-Jakob disease patients
Author: Schmitz, Matthias
Llorens Torres, Franc
Pracht, Alexander
Thom, Tobias
Correia, Ângela
Zafar, Saima
Ferrer, Isidro (Ferrer Abizanda)
Zerr, Inga
Keywords: Malaltia de Creutzfeldt-Jakob
Marcadors bioquímics
Creutzfeldt-Jakob disease
Biochemical markers
Issue Date: 1-Nov-2016
Publisher: Impact Journals
Abstract: The identification of reliable diagnostic biomarkers in differential diagnosis of neurodegenerative diseases is an ongoing topic. A previous two-dimensional proteomic study on cerebrospinal fluid (CSF) revealed an elevated level of an enzyme, mitochondria! malate dehydrogenase 1 (MDH1), in sporadic Creutzfeldt-Jakob disease (sCJD) patients. Here, we could demonstrate the expression of MDH1 in neurons as well as in the neuropil. Its levels are lower in sCJD brains than in control brains. An examination of CSF-MDH1 in sCJD patients by ELISA revealed a significant elevation of CSF-MDH1 levels in sCJD patients (independently from the PRNP codon 129 MV genotype or the prion protein scrapie (PrPsc) type) in comparison to controls. In combination with total tau (tau), CSF-MDH1 detection exhibited a high diagnostic accuracy for sCJD diagnosis with a sensitivity of 97.5% and a specificity of 95.6%. A correlation study of MDH1 level in CSF with other neurodegenerative marker proteins revealed a significant positive correlation between MDH1 concentration with tau, 14-3-3 and neuron specific enolase level. In conclusion, our study indicated the potential of MDH1 in combination with tau as an additional biomarker in sCJD improving diagnostic accuracy of tau markedly.
Note: Reproducció del document publicat a: https://doi.org/10.18632/aging.101101
It is part of: Aging, 2016, vol. 8, num. 11, p. 2927-2935
URI: http://hdl.handle.net/2445/125364
Related resource: https://doi.org/10.18632/aging.101101
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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