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Title: C-terminal fragments of the amyloid precursor protein in cerebrospinal fluid as potential biomarkers for Alzheimer disease
Author: García Ayllón, María Salud
Lopez Font, Inmaculada
Boix, Claudia P.
Fortea Ormaechea, Juan
Sánchez del Valle Díaz, Raquel
Lleó Bisa, Alberto
Molinuevo, José L.
Zetterberg, Henrik
Blennow, Kaj
Sáez Valero, Javier
Keywords: Malaltia d'Alzheimer
Marcadors bioquímics
Alzheimer's disease
Biochemical markers
Issue Date: May-2017
Publisher: Nature Publishing Group
Abstract: This study assesses whether C-terminal fragments (CTF) of the amyloid precursor protein (APP) are present in cerebrospinal fluid (CSF) and their potential as biomarkers for Alzheimer's disease (AD). Immunoprecipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging with specific antibodies against particular domains served to characterize CTFs of APP in human CSF. We demonstrate that APP-CTFs are detectable in human CSF, being the most abundant a 25-kDa fragment, probably resulting from proteolytic processing by η-secretase. The level of the 25-kDa APP-CTF was evaluated in three independent CSF sample sets of patients and controls. The CSF level of this 25-kDa CTF is higher in subjects with autosomal dominant AD linked to PSEN1 mutations, in demented Down syndrome individuals and in sporadic AD subjects compared to age-matched controls. Our data suggest that APP-CTF could be a potential diagnostic biomarker for AD.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2017, vol. 7, num. 1, p. 2477
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

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