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http://hdl.handle.net/2445/125936| Title: | PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts |
| Author: | Garcia i Molleví, David Aytés Meneses, Álvaro Berdiel Acer, Mireia Padullés Mosella, Laura Martínez Iniesta, María Sanjuan, Xavier Salazar Soler, Ramón Villanueva Garatachea, Alberto |
| Keywords: | Càncer Fibroblasts Metàstasi Cancer Fibroblasts Metastasis |
| Issue Date: | 8-Jul-2009 |
| Publisher: | BioMed Central |
| Abstract: | We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel of colorectal and pancreatic cell lines in CM from CAFlm resulted in overexpression of mRNA PRL-3 and higher overexpression in CAFs than in non-activated fibroblasts. Moreover PRL-3 mRNA expression correlates with expression of alpha-SMA and deposition of collagen fibrils in the stroma. We demonstrate that products secreted by CAFs trigger PRL-3 overexpression in cancer cells. Identification of these factors may contribute to new stroma-targeted therapies for desmoplastic tumours |
| Note: | Reproducció del document publicat a: https://doi.org/10.1186/1476-4598-8-46 |
| It is part of: | Molecular Cancer, 2009, vol. 8, p. 46 |
| URI: | http://hdl.handle.net/2445/125936 |
| Related resource: | https://doi.org/10.1186/1476-4598-8-46 |
| ISSN: | 1476-4598 |
| Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Ciències Clíniques) |
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