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Title: | Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci |
Author: | Rodríguez-Rubio, Lorena Chang, Wai-Ling Gutiérrez, Diana Lavigne, Rob Martínez, Beatriz Rodríguez, Ana Govers, Sander K. Aertsen, Abram Hirl, Christine Biebl, Manfred Briers, Yves García, Pilar |
Keywords: | Estreptococs Enzims microbians Bacteriòfags Streptococcus Microbial enzymes Bacteriophages |
Issue Date: | 24-Oct-2016 |
Publisher: | Nature Publishing Group |
Abstract: | Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10min. In addition, lower doses of Art240 are required to achieve the same bactericidal effect. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/srep35382 |
It is part of: | Scientific Reports, 2016, vol. 6, num. 35382 |
URI: | http://hdl.handle.net/2445/125974 |
Related resource: | https://doi.org/10.1038/srep35382 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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