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Title: | RSR-2, the Caenorhabditis elegans Ortholog of Human Spliceosomal Component SRm300/SRRM2, Regulates Development by Influencing the Transcriptional Machinery |
Author: | Fontrodona, Laura Porta de la Riva, Montserrat Morán, Tomás Niu, Wei Diaz, Mònica Aristizábal Corrales, David Villanueva Garatachea, Alberto Schwartz Navarro, Simó Reinke, Valerie Cerón Madrigal, Julián |
Keywords: | Expressió gènica Cromatina Gene expression Chromatin |
Issue Date: | 6-Jun-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Protein components of the spliceosome are highly conserved in eukaryotes and can influence several steps of the gene expression process. RSR-2, the Caenorhabditis elegans ortholog of the human spliceosomal protein SRm300/SRRM2, is essential for viability, in contrast to the yeast ortholog Cwc21p. We took advantage of mutants and RNA interference (RNAi) to study rsr-2 functions in C. elegans, and through genetic epistasis analysis found that rsr-2 is within the germline sex determination pathway. Intriguingly, transcriptome analyses of rsr-2(RNAi) animals did not reveal appreciable splicing defects but instead a slight global decrease in transcript levels. We further investigated this effect in transcription and observed that RSR-2 colocalizes with DNA in germline nuclei and coprecipitates with chromatin, displaying a ChIP-Seq profile similar to that obtained for the RNA Polymerase II (RNAPII). Consistent with a novel transcription function we demonstrate that the recruitment of RSR-2 to chromatin is splicing-independent and that RSR-2 interacts with RNAPII and affects RNAPII phosphorylation states. Proteomic analyses identified proteins associated with RSR-2 that are involved in different gene expression steps, including RNA metabolism and transcription with PRP-8 and PRP-19 being the strongest interacting partners. PRP-8 is a core component of the spliceosome and PRP-19 is the core component of the PRP19 complex, which interacts with RNAPII and is necessary for full transcriptional activity. Taken together, our study proposes that RSR-2 is a multifunctional protein whose role in transcription influences C. elegans development. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pgen.1003543 |
It is part of: | PLoS Genetics, 2013, vol. 9, num. 6, p. e1003543 |
URI: | http://hdl.handle.net/2445/126462 |
Related resource: | https://doi.org/10.1371/journal.pgen.1003543 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Publicacions de projectes de recerca finançats per la UE |
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