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http://hdl.handle.net/2445/126528
Title: | NuRD-dependent DNA methylation prevents ES cells from accessing a trophectoderm fate |
Author: | Latos, Paulina A. Helliwell, Christine Mosaku, Olukunbi Dudzinska, Dominika A. Stubbs, Bryony Berdasco, María Esteller, Manel Hendrich, Brian |
Keywords: | Cèl·lules mare ADN Stem cells DNA |
Issue Date: | 15-Apr-2012 |
Publisher: | The Company of Biologists |
Abstract: | Embryonic Stem (ES) cells are able to give rise to the three germ layers of the embryo but are prevented from contributing to the trophoblast. The molecular nature of this barrier between embryonic and trophectodermal cell fates is not clear, but is known to involve DNA methylation. Here we demonstrate that the Nucleosome Remodeling and Deacetylation (NuRD) co-repressor complex maintains the developmental barrier between embryonic and trophectodermal cell fates by maintaining transcriptional silencing of trophectoderm determinant genes in ES cells. We further show that NuRD activity facilitates DNA methylation of several of its target promoters, where it acts non-redundantly with DNA methylation to enforce transcriptional silencing. NuRD-deficient ES cells fail to completely silence expression of the trophectoderm determinant genes Elf5 and Eomes, but this alone is not sufficient to induce transdifferentiation towards the trophectoderm fate. Rather this leaves ES cells capable of activating expression of trophectoderm-specific genes in response to appropriate extracellular signals, enabling them to commit to a trophectodermal cell fate. Our findings clarify the molecular nature of the developmental barrier between the embryonic and trophoblast cell fates, and establish a role for NuRD activity in specifying sites for de novo DNA methylation. (C) 2012. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
Note: | Reproducció del document publicat a: https://doi.org/10.1242/bio.2012513 |
It is part of: | Biology Open, 2012, vol. 1, num. 4, p. 341-352 |
URI: | http://hdl.handle.net/2445/126528 |
Related resource: | https://doi.org/10.1242/bio.2012513 |
Appears in Collections: | Publicacions de projectes de recerca finançats per la UE Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Ciències Fisiològiques) |
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