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Title: | Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes |
Author: | Rodríguez Ubreva, Javier Ciudad, Laura Gómez Cabrero, David Parra Bola, Mª Isabel Bussmann, Lars H. Tullio, Alessandro di Kallin, Eric M. Tegnér, Jesper Graf, Thomas Ballestar Tarín, Esteban |
Keywords: | ADN Cèl·lules B DNA B cells |
Issue Date: | 1-Mar-2012 |
Publisher: | Oxford University Press |
Abstract: | Transcription factor-induced lineage reprogramming or transdifferentiation experiments are essential for understanding the plasticity of differentiated cells. These experiments helped to define the specific role of transcription factors in conferring cell identity and played a key role in the development of the regenerative medicine field. We here investigated the acquisition of DNA methylation changes during C/EBP alpha-induced pre-B cell to macrophage transdifferentiation. Unexpectedly, cell lineage conversion occurred without significant changes in DNA methylation not only in key B cell- and macrophage-specific genes but also throughout the entire set of genes differentially methylated between the two parental cell types. In contrast, active and repressive histone modification marks changed according to the expression levels of these genes. We also demonstrated that C/EBP alpha and RNA Pol II are associated with the methylated promoters of macrophage-specific genes in reprogrammed macrophages without inducing methylation changes. Our findings not only provide insights about the extent and hierarchy of epigenetic events in pre-B cell to macrophage transdifferentiation but also show an important difference to reprogramming towards pluripotency where promoter DNA demethylation plays a pivotal role. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkr1015 |
It is part of: | Nucleic Acids Research, 2012, vol. 40, num. 5, p. 1954-1968 |
URI: | http://hdl.handle.net/2445/126530 |
Related resource: | https://doi.org/10.1093/nar/gkr1015 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Publicacions de projectes de recerca finançats per la UE |
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