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http://hdl.handle.net/2445/126548
Title: | Standardized nanomechanical atomic force microscopy procedure (SNAP) for measuring soft and biological samples |
Author: | Schillers, Hermann Rianna, Carmela Schape, Jens Luque González, Tomás Doschke, Holger Walte, Mike Uriarte, Juan José Campillo, Noelia Michanetzis, Georgios P. A. Bobrowska, Justyna Dumitru, Andra Herruzo, Elena T. Bovio, Simone Parot, Pierre Galluzzi, Massimiliano Podestà, Alessandro Puricelli, Luca Scheuring, Simon Missirlis, Yannis Garcia, Ricardo Odorico, Michael Teulon, Jean-Marie Lafont, Frank Lekka, Malgorzata Rico Camps, Félix Rigato, Annafrancesca Pellequer, Jean-Luc Oberleithner, Hans Navajas Navarro, Daniel Radmacher, Manfred |
Keywords: | Microscòpia de força atòmica Nanotecnologia Biomecànica Atomic force microscopy Nanotechnology Biomechanics |
Issue Date: | 11-Jul-2017 |
Publisher: | Nature Publishing Group |
Abstract: | We present a procedure that allows a reliable determination of the elastic (Young's) modulus of soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM procedure (SNAP) ensures the precise adjustment of the AFM optical lever system, a prerequisite for all kinds of force spectroscopy methods, to obtain reliable values independent of the instrument, laboratory and operator. Measurements of soft hydrogel samples with a well-defined elastic modulus using different AFMs revealed that the uncertainties in the determination of the deflection sensitivity and subsequently cantilever's spring constant were the main sources of error. SNAP eliminates those errors by calculating the correct deflection sensitivity based on spring constants determined with a vibrometer. The procedure was validated within a large network of European laboratories by measuring the elastic properties of gels and living cells, showing that its application reduces the variability in elastic moduli of hydrogels down to 1%, and increased the consistency of living cells elasticity measurements by a factor of two. The high reproducibility of elasticity measurements provided by SNAP could improve significantly the applicability of cell mechanics as a quantitative marker to discriminate between cell types and conditions. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-05383-0 |
It is part of: | Scientific Reports, 2017, vol. 7 |
URI: | http://hdl.handle.net/2445/126548 |
Related resource: | https://doi.org/10.1038/s41598-017-05383-0 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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