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http://hdl.handle.net/2445/126612
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DC Field | Value | Language |
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dc.contributor.author | Abate Daga, Daniel | - |
dc.contributor.author | Andreu, Nuria | - |
dc.contributor.author | Camacho Sánchez, Juan Miguel | - |
dc.contributor.author | Alemany Bonastre, Ramon | - |
dc.contributor.author | Herance, Raúl | - |
dc.contributor.author | Millan, Olga | - |
dc.contributor.author | Fillat i Fonts, Cristina | - |
dc.date.accessioned | 2018-11-30T10:05:26Z | - |
dc.date.available | 2018-11-30T10:05:26Z | - |
dc.date.issued | 2011-10-18 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126612 | - |
dc.description.abstract | Replication-competent adenoviruses armed with thymidine kinase (TK) combine the concepts of virotherapy and suicide gene therapy. Moreover TK-activity can be detected by noninvasive positron emission-computed tomography (PET) imaging, what could potentially facilitate virus monitoring in vivo. Here, we report the generation of a novel oncolytic adenovirus that incorporates the Tat8-TK gene under the control of the Major Late Promoter in a highly selective backbone thus providing selectivity by targeting the retinoblastoma pathway. The selective oncolytic TK virus, termed ICOVIR5-TK-L, showed reduced potency compared to a non-selective counterpart. However the combination of ICOVIR5-TK-L with ganciclovir (GCV) induced a potent antitumoural effect similar to that of wild type adenovirus in a preclinical model of pancreatic cancer. Although the treatment with GCV provoked a reduction in the viral yield, both in vitro and in vivo, a two-cycle treatment of virus and GCV resulted in an enhanced antitumoral response that correlated with high TK-activity, based on microPET measurements. Thus, TK-expressing oncolytic adenoviruses can be traced by PET imaging providing real time information on the activity of the virus and its antitumoral potency can be optimized by GCV dosing. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0026142 | - |
dc.relation.ispartof | PloS One, 2011, vol. 6, num. 10, p. e26142 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0026142 | - |
dc.rights | cc by (c) Abate Daga et al., 2011 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Adenovirus | - |
dc.subject.classification | Càncer | - |
dc.subject.other | Adenoviruses | - |
dc.subject.other | Cancer | - |
dc.title | Oncolytic Adenoviruses Armed with Thymidine Kinase Can Be Traced by PET Imaging and Show Potent Antitumoural Effects by Ganciclovir Dosing | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2018-07-24T12:58:49Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22028820 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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Abate-DagaD.pdf | 2.55 MB | Adobe PDF | View/Open |
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