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Title: Inflammatory dysregulation of monocytes in pediatric patients with obsessive-compulsive disorder
Author: Rodríguez Ferret, Natalia
Morer Liñán, Astrid
González-Navarro, Europa Azucena
Serra Pagès, Carles
Boloc, Daniel
Torres, Teresa
García Cerro, Susana
Mas Herrero, Sergi
Gassó Astorga, Patricia
Lázaro García, Luisa
Keywords: Psiquiatria infantil
Neurosi obsessiva
Child psychiatry
Obsessive-compulsive disorder
Issue Date: 28-Dec-2017
Publisher: BioMed Central
Abstract: BACKGROUND: Although the exact etiology of obsessive-compulsive disorder (OCD) is unknown, there is growing evidence of a role for immune dysregulation in the pathophysiology of the disease, especially in the innate immune system including the microglia. To test this hypothesis, we studied inflammatory markers in monocytes from pediatric patients with OCD and from healthy controls. METHODS: We determined the percentages of total monocytes, CD16+ monocytes, and classical (CD14highCD16-), intermediate (CD14highCD16low), and non-classical (CD14lowCD16high) monocyte subsets in 102 patients with early-onset OCD and in 47 healthy controls. Moreover, proinflammatory cytokine production (GM-CSF, IL-1β, IL-6, IL-8, and TNF-α) was measured by multiplex Luminex analysis in isolated monocyte cultures, in basal conditions, after exposure to lipopolysaccharide (LPS) to stimulate immune response or after exposure to LPS and the immunosuppressant dexamethasone. RESULTS: OCD patients had significantly higher percentages of total monocytes and CD16+ monocytes than healthy controls, mainly due to an increase in the intermediate subset but also in the non-classical monocytes. Monocytes from OCD patients released higher amounts of GM-CSF, IL-1β, IL-6, IL-8, and TNF-α than healthy controls after exposure to LPS. However, there were no significant differences in basal cytokine production or the sensitivity of monocytes to dexamethasone treatment between both groups. Based on monocyte subset distribution and cytokine production after LPS stimulation, patients receiving psychoactive medications seem to have an intermediate inflammatory profile, that is, lower than non-medicated OCD individuals and higher than healthy controls. CONCLUSIONS: These results strongly support the involvement of an enhanced proinflammatory innate immune response in the etiopathogenesis of early-onset OCD.
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It is part of: Journal of Neuroinflammation, 2017, vol. 14, num. 1, p. 261
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ISSN: 1742-2094
Appears in Collections:Articles publicats en revistes (Medicina)
Articles publicats en revistes (Fonaments Clínics)

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