Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/127349
Title: | Lighting up G protein-coupled purinergic receptors with engineered fluorescent ligands |
Author: | Ciruela Alférez, Francisco Fernández Dueñas, Víctor Jacobson, Kenneth A. |
Keywords: | Proteïnes G Receptors de neurotransmissors Neurofarmacologia Oligòmers G Proteins Neurotransmitter receptors Neuropharmacology Oligomers |
Issue Date: | 16-Apr-2015 |
Publisher: | Elsevier Ltd |
Abstract: | The use of G protein-coupled receptors fluorescent ligands is undergoing continuous expansion. In line with this, fluorescent agonists and antagonists of high affinity for G protein-coupled adenosine and P2Y receptors have been shown to be useful pharmacological probe compounds. Fluorescent ligands for A1R, A2AR, and A3R (adenosine receptors) and P2Y2R, P2Y4R, P2Y6R, and P2Y14R (nucleotide receptors) have been reported. Such ligands have been successfully applied to drug discovery and to GPCR characterization by flow cytometry, fluorescence correlation spectroscopy, fluorescence microscopy, fluorescence polarization, fluorescence resonance energy transfer and scanning confocal microscopy. Here we summarize recently reported and readily available representative fluorescent ligands of purinergic receptors. In addition, we pay special attention on the use of this family of fluorescent ligands revealing two main aspects of purinergic receptor biology, namely ligand binding and receptor oligomerization. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.neuropharm.2015.04.001 |
It is part of: | Neuropharmacology, 2015, vol. 98, p. 58-67 |
URI: | http://hdl.handle.net/2445/127349 |
Related resource: | https://doi.org/10.1016/j.neuropharm.2015.04.001 |
ISSN: | 0028-3908 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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