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http://hdl.handle.net/2445/127915| Title: | Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure |
| Author: | Benabou Zdaou, Sanae Garavís, Miguel Lyonnais, Sébastien Eritja i Casadellà, Ramon González, Carlos Gargallo Gómez, Raimundo |
| Keywords: | ADN Espectroscòpia de ressonància magnètica nuclear Cromatografia de permeació per gel Electroforesi DNA Nuclear magnetic resonance spectroscopy Gel permeation chromatography Electrophoresis |
| Issue Date: | 9-Feb-2016 |
| Publisher: | Royal Society of Chemistry |
| Abstract: | The nature and length of loops connecting cytosine tracts in i-motif structures may affect their stability. In this work, the influence of the nature of the nucleobases located in two of the loops of an intramolecular i-motif is studied using spectroscopy, separation techniques, and multivariate data analysis. Insertion of bases other than thymine induces an additional acid-base equilibrium with pKa~4.5. The presence of two guanine bases in the loops, placed opposite to each other, decreases the thermal stability of the structure. In contrast, thymine and cytosine bases in these positions stabilize the structure |
| Note: | Versió postprint del document publicat a: https://doi.org/10.1039/C5CP07428B |
| It is part of: | Physical Chemistry Chemical Physics, 2016, vol. 18, num. 11, p. 7997-8004 |
| URI: | http://hdl.handle.net/2445/127915 |
| Related resource: | https://doi.org/10.1039/C5CP07428B |
| ISSN: | 1463-9076 |
| Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 656926.pdf | 857.8 kB | Adobe PDF | View/Open |
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