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http://hdl.handle.net/2445/128079
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DC Field | Value | Language |
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dc.contributor.author | Wolfenson, Haguy | - |
dc.contributor.author | Meacci, Giovanni | - |
dc.contributor.author | Liu, Shuaimin | - |
dc.contributor.author | Stachowiak, Matthew R. | - |
dc.contributor.author | Iskratsch, Thomas | - |
dc.contributor.author | Ghassemi, Saba | - |
dc.contributor.author | Roca-Cusachs Soulere, Pere | - |
dc.contributor.author | Shaughnessy, Ben O' | - |
dc.contributor.author | Hone, James | - |
dc.contributor.author | Sheetz, Michael P. | - |
dc.date.accessioned | 2019-02-08T14:57:22Z | - |
dc.date.available | 2019-02-08T14:57:22Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1465-7392 | - |
dc.identifier.uri | http://hdl.handle.net/2445/128079 | - |
dc.description.abstract | Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1038/ncb3277 | - |
dc.relation.ispartof | Nature Cell Biology, 2016, vol. 18, p. 33-42 | - |
dc.relation.uri | https://doi.org/10.1038/ncb3277 | - |
dc.rights | (c) Wolfenson, Haguy et al., 2016 | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Matriu extracel·lular | - |
dc.subject.other | Extracellular matrix | - |
dc.title | Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 660367 | - |
dc.date.updated | 2019-02-08T14:57:22Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/332045/EU//RIGIDITY SENSING | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26619148 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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660367.pdf | 988.17 kB | Adobe PDF | View/Open |
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