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http://hdl.handle.net/2445/128128
Title: | Evaluation of anti-inflammatory activity of food compounds using zebrafish |
Author: | Arteaga, Cristina Boix Sabrià, Núria Barenys Espadaler, Marta Llobet Mallafré, Joan M. (Joan Maria) Gómez Catalán, Jesús |
Keywords: | Peix zebra Toxicologia Zebra danio Toxicology |
Issue Date: | 2018 |
Publisher: | Research Signpost |
Abstract: | The principal aim of this work was to optimize and apply a zebrafish experimental model for the screening of anti-inflammatory substances present in the Mediterranean diet. The zebrafish is an organism widely used in various fields of experimental biology. The inflammation is easily inducible, reproducible and visualized in their early stages of development. Specifically, the migration of neutrophils to the injured caudal fin, one of the first steps of the inflammatory response, is quantitatively measured by image analysis. The anti-inflammatory effect of natural compounds can be evaluated as a decrease of migration. Adverse effects triggered by inflammation are mainly mediated by reactive oxygen species. The anti-oxidant activity of compounds was evaluated in zebrafish embryo measuring their protective effect against tert-butyl hydroperoxide toxicity. Several phenolic compounds have been assayed. Our results showed that the compounds with the greatest decrease on neutrophil migration were chlorogenic acid and cyanidin. The activity of these two polyphenols was quite similar to that observed with anti-inflammatory drugs (indomethacin, piroxicam) and NADPH oxidase inhibitor compounds (dibenzoidolium, apocynin). The anti-inflammatory and the anti-oxidant activity of the assayed polyphenols did not show a clear correlation. |
Note: | Podeu consultar el llibre complet a: http://hdl.handle.net/2445/128014 |
Note: | Reproducció del capítol del llibre publicat a: http://www.trnres.com/ebookcontents.php?id=287 |
It is part of: | Recent Advances in Pharmaceutical Sciences VIII, 2018, Research Signpost. Editors: Diego Muñoz-Torrero, Yolanda Cajal and Joan Maria Llobet. ISBN: 978-81-308-0579-5. Chapter 5, p. 79-94 |
URI: | http://hdl.handle.net/2445/128128 |
Related resource: | http://hdl.handle.net/2445/128014 http://hdl.handle.net/2445/116442 |
Appears in Collections: | Llibres / Capítols de llibre (Farmacologia, Toxicologia i Química Terapèutica) |
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