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http://hdl.handle.net/2445/128159
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DC Field | Value | Language |
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dc.contributor.author | Agramunt, Jordi | - |
dc.contributor.author | Pedroso Muller, Enrique | - |
dc.contributor.author | Kreda, Silvia M. | - |
dc.contributor.author | Juliano, Rudolph L. | - |
dc.contributor.author | Grandas Sagarra, Anna | - |
dc.date.accessioned | 2019-02-12T11:32:24Z | - |
dc.date.available | 2019-02-12T11:32:24Z | - |
dc.date.issued | 2019-02-06 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://hdl.handle.net/2445/128159 | - |
dc.description.abstract | Addition of small molecule Retro-1 has been described to enhance antisense and splice switching oligonucleotides. With the aim of assessing the effect of covalently linking Retro-1 to the biologically active oligonucleotide, three different derivatives of Retro-1 were prepared that incorporated a phosphoramidite group, a thiol or a 1,3-diene, respectively. Retro-1-oligonucleotide conjugates were assembled both on-resin (coupling of the phosphoramidite) and from reactions in solution (Michael-type thiol-maleimide reaction and Diels-Alder cycloaddition). Splice switching assays with the resulting conjugates showed that they were active but that they provided little advantage over the unconjugated oligonucleotide in the well-known HeLa Luc705 reporter system. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/molecules24030579 | - |
dc.relation.ispartof | Molecules, 2019, vol. 24, num. 3, p. 579 | - |
dc.relation.uri | https://doi.org/10.3390/molecules24030579 | - |
dc.rights | cc-by (c) Agramunt, Jordi et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Oligonucleòtids | - |
dc.subject.classification | Síntesi orgànica | - |
dc.subject.other | Oligonucleotides | - |
dc.subject.other | Organic synthesis | - |
dc.title | Retro-1-oligonucleotide conjugates. Synthesis and biological evaluation | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 685350 | - |
dc.date.updated | 2019-02-12T11:32:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30736307 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) |
Files in This Item:
File | Description | Size | Format | |
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685350.pdf | 2.9 MB | Adobe PDF | View/Open |
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