Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/129473
Title: Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing
Author: Perea Gil, Isaac
Gálvez Montón, Carolina
Prat Vidal, Cristina
Jorba, Ignasi
Segú Vergés, Cristina
Roura, Santiago
Soler Botija, Carolina
Iborra Egea, Oriol
Revuelta López, Elena
Fernández, Marco A.
Farré Ventura, Ramon
Navajas Navarro, Daniel
Bayes Genis, Antoni
Keywords: Infart de miocardi
Malalties coronàries
Terapèutica
Enginyeria de proteïnes
Myocardial infarction
Coronary diseases
Therapeutics
Protein Engineering
Issue Date: 30-Apr-2018
Publisher: Nature Publishing Group
Abstract: Cardiac tissue engineering, which combines cells and supportive scaffolds, is an emerging treatment for restoring cardiac function after myocardial infarction (MI), although, the optimal construct remains a challenge. We developed two engineered cardiac grafts, based on decellularized scaffolds from myocardial and pericardial tissues and repopulated them with adipose tissue mesenchymal stem cells (ATMSCs). The structure, macromechanical and micromechanical scaffold properties were preserved upon the decellularization and recellularization processes, except for recellularized myocardium micromechanics that was ∼2-fold stiffer than native tissue and decellularized scaffolds. Proteome characterization of the two acellular matrices showed enrichment of matrisome proteins and major cardiac extracellular matrix components, considerably higher for the recellularized pericardium. Moreover, the pericardial scaffold demonstrated better cell penetrance and retention, as well as a bigger pore size. Both engineered cardiac grafts were further evaluated in pre-clinical MI swine models. Forty days after graft implantation, swine treated with the engineered cardiac grafts showed significant ventricular function recovery. Irrespective of the scaffold origin or cell recolonization, all scaffolds integrated with the underlying myocardium and showed signs of neovascularization and nerve sprouting. Collectively, engineered cardiac grafts -with pericardial or myocardial scaffolds- were effective in restoring cardiac function post-MI, and pericardial scaffolds showed better structural integrity and recolonization capability.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-25115-2
It is part of: Scientific Reports, 2018, vol. 8, num. 6708
URI: http://hdl.handle.net/2445/129473
Related resource: https://doi.org/10.1038/s41598-018-25115-2
ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Biomedicina)

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