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http://hdl.handle.net/2445/130097
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DC Field | Value | Language |
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dc.contributor.author | Sigdel, Tara K. | - |
dc.contributor.author | Bestard Matamoros, Oriol | - |
dc.contributor.author | Salomonis, Nathan | - |
dc.contributor.author | Hsieh, Szu-Chuan | - |
dc.contributor.author | Torras Ambròs, Joan | - |
dc.contributor.author | Naesens, Maarten | - |
dc.contributor.author | Tran, Tim Q. | - |
dc.contributor.author | Roedder, Silke | - |
dc.contributor.author | Sarwal, Minnie M. | - |
dc.date.accessioned | 2019-03-12T11:16:56Z | - |
dc.date.available | 2019-03-12T11:16:56Z | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 0041-1337 | - |
dc.identifier.uri | http://hdl.handle.net/2445/130097 | - |
dc.description.abstract | Background: polyomavirus nephropathy (PVAN) is a common cause of kidney allograft dysfunction and loss. To identify PVAN-specific gene expression and underlying molecular mechanisms, we analyzed kidney biopsies with and without PVAN. Methods: the study included 168 posttransplant renal allograft biopsies (T cell-mediated rejection [TCMR] = 26, PVAN = 10, normal functioning graft = 73, and interstitial fibrosis/tubular atrophy = 59) from 168 unique kidney allograft recipients. We performed gene expression assays and bioinformatics analysis to identify a set of PVAN-specific genes. Validity and relevance of a subset of these genes are validated by quantitative polymerase chain reaction and immunohistochemistry. Results: unsupervised hierarchical clustering analysis of all the biopsies revealed high similarity between PVAN and TCMR gene expression. Increased statistical stringency identified 158 and 252 unique PVAN and TCMR injury-specific gene transcripts respectively. Although TCMR-specific genes were overwhelmingly involved in immune response costimulation and TCR signaling, PVAN-specific genes were mainly related to DNA replication process, RNA polymerase assembly, and pathogen recognition receptors. A principal component analysis (PCA) using these genes further confirmed the most optimal separation between the 3 different clinical phenotypes. Validation of 4 PVAN-specific genes (RPS15, complement factor D, lactotransferrin, and nitric oxide synthase interacting protein) by quantitative polymerase chain reaction and confirmation by immunohistochemistry of 2 PVAN-specific proteins with antiviral function (lactotransferrin and IFN-inducible transmembrane 1) was done. Conclusions: in conclusion, even though PVAN and TCMR kidney allografts share great similarities on gene perturbation, PVAN-specific genes were identified with well-known antiviral properties that provide tools for discerning PVAN and AR as well as attractive targets for rational drug design. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Lippincott, Williams & Wilkins | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1097/TP.0000000000001214 | - |
dc.relation.ispartof | Transplantation, 2016, vol. 100, num. 10, p. 2062-2070 | - |
dc.relation.uri | https://doi.org/10.1097/TP.0000000000001214 | - |
dc.rights | (c) Lippincott, Williams & Wilkins, 2016 | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Antígens | - |
dc.subject.classification | Genètica | - |
dc.subject.classification | Malalties del ronyó | - |
dc.subject.classification | Trasplantament renal | - |
dc.subject.classification | Efectes secundaris | - |
dc.subject.classification | Infeccions | - |
dc.subject.other | Antigens | - |
dc.subject.other | Genetics | - |
dc.subject.other | Kidney diseases | - |
dc.subject.other | Kidney transplantation | - |
dc.subject.other | Side effects | - |
dc.subject.other | Infections | - |
dc.title | Intragraft antiviral-specific gene expression as a distinctive transcriptional signature for studies in polyomavirus-associated nephropathy | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 665166 | - |
dc.date.updated | 2019-03-12T11:16:56Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27140517 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Ciències Clíniques) |
Files in This Item:
File | Description | Size | Format | |
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665166.pdf | 896.98 kB | Adobe PDF | View/Open |
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