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Title: Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
Author: Scholz, Gregor
Mariana, Shinta
Dharmawan, Agus Budi
Syamsu, Iqbal
Hörmann, Philipp
Reuse, Carsten
Hartmann, Jana
Hiller, Karsten
Prades García, Juan Daniel
Wasisto, Hutomo Suryo
Waag, Andreas
Keywords: Díodes electroluminescents
Dispositius optoelectrònics
Cultiu cel·lular
Light emitting diodes
Optoelectronic devices
Cell culture
Issue Date: 11-Mar-2019
Publisher: MDPI
Abstract: Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.
Note: Reproducció del document publicat a:
It is part of: Sensors, 2019, vol. 19, num. 5, p. 1234-1247
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ISSN: 1424-8220
Appears in Collections:Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Publicacions de projectes de recerca finançats per la UE

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