Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/130296
Title: | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
Author: | Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades García, Juan Daniel Wasisto, Hutomo Suryo Waag, Andreas |
Keywords: | Díodes electroluminescents Dispositius optoelectrònics Cultiu cel·lular Light emitting diodes Optoelectronic devices Cell culture |
Issue Date: | 11-Mar-2019 |
Publisher: | MDPI |
Abstract: | Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/s19051234 |
It is part of: | Sensors, 2019, vol. 19, num. 5, p. 1234-1247 |
URI: | http://hdl.handle.net/2445/130296 |
Related resource: | https://doi.org/10.3390/s19051234 |
ISSN: | 1424-8220 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) Publicacions de projectes de recerca finançats per la UE |
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689238.pdf | 5.28 MB | Adobe PDF | View/Open |
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