Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/130339
Title: Effects of two different decellularization routes on the mechanical properties of decellularized lungs
Author: Urbano, Jessica Julioti
Palma, Renata Kelly da
Lima, Flavia Mafra de
Fratini, Paula
Lopes Guimaraes, Leticia
Uriarte, Juan J.
Heineck Alvarenga, Leticia
Miglino, Maria Angelica
Paula Vieira, Rodolfo de
Araujo Prates, Renato
Navajas Navarro, Daniel
Farré Ventura, Ramon
Franco Oliveira, Luis Vicente
Keywords: Pulmó
Bioenginyeria
Models animals en la investigació
Lung
Bioengineering
Animal models in research
Issue Date: 1-Jun-2017
Publisher: Public Library of Science (PLoS)
Abstract: Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process. This study was performed by using the lungs excised from 30 healthy male C57BL/6 mice, which were divided into 3 groups: tracheal decellularization (TDG), perfusion decellularization (PDG), and control groups (CG). Both decellularized groups were subjected to decellularization protocol with a solution of 1% sodium dodecyl sulfate. The behaviour of mechanical properties of the acellular lungs was measured after decellularization process. Static (Est) and dynamic (Edyn) elastances were obtained by the end-inspiratory occlusion method. TDG and PDG showed reduced Est and Edyn elastances after lung decellularization. Scanning electron microscopy showed no structural changes after lung decellularization of the TDG and PDG. In conclusion, was demonstrated that there is no significant difference in the behaviour of mechanical properties and extracellular matrix of the decellularized lungs by using two different routes through the trachea and pulmonary artery.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0178696
It is part of: PLoS One, 2017, vol. 12, num. 6, p. e0178696
URI: http://hdl.handle.net/2445/130339
Related resource: https://doi.org/10.1371/journal.pone.0178696
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biomedicina)

Files in This Item:
File Description SizeFormat 
676590.pdf973.82 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons