Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/130524
Title: | Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation |
Author: | Gandolfini, Ilaria Crespo, Elena Baweja, Mukta Jarque, Marta Donadei, Chiara Luque, Sergio Montero Pérez, Núria Allesina, Anna Perin, Laura Maggiore, Umberto Cravedi, Paolo Bestard Matamoros, Oriol |
Keywords: | Trasplantament renal Immunologia de la trasplantació Malalties del ronyó Kidney transplantation Transplantation immunology Kidney diseases |
Issue Date: | 30-Jul-2018 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Donor-specific (d-sp) interferon gamma enzyme-linked immunosorbent spot (d-sp ELISPOT)and Panel of reactive T-cell (PRT) ELISPOT assays have been developed to detect alloreactive memory T (Tmem) cells in order to estimate the risk of acute rejection after kidney transplantation. Adding IL15 to the PRT assay (PRT+IL15) may uncover the presence of pathogenic alloreactive CD28-Tmem. Face-to-face comparisons of these assays have not been done yet. We performed pre-transplant d-sp ELISPOT and PRT assays (±IL15, against six B-cell lines) in 168 consecutive kidney transplant recipients and evaluated the multivariable-adjusted associations with biopsy-proven acute rejection (BPAR), de novo donor-specific antibodies (DSA), and eGFR decline over a 48-month follow-up period. D-sp ELISPOT was positive in 81 (48%) subjects, while 71 (42%) and 81 (48%) subjects displayed positive PRT and PRT+IL15, respectively.Their median [interquartile range] numerical test result was 23 [6±65], 18 [8±37], and 26 [10±45] spots/3x105 PBMCs, respectively. The number of PRT spots were weakly correlated with those of d-sp ELISPOT, but highly correlated with PRT+IL15 (rho = 0.96, P<0.001). d-sp ELISPOT, but not PRT (±IL15) was independently associated with BPAR (adjusted Odds Ratio of BPAR associated with d-sp ELISPOT positivity: 4.20 [95%CI: 1.06 to 21.73; P = 0.041]). Unlike d-sp ELISPOT, median PRT and PRT+IL15 were independently associated with higher Δ3-48month eGFR decline post-transplantation (for both assays, about -3mL/min/1.73m2 per one standard deviation unit increase in the spot number). Pre-transplant T-cell immune-monitoring using d-sp ELISPOT and PRT assays identifies kidney transplant candidates at high risk of BPAR and worse kidney allograft progression. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0200696 |
It is part of: | PLoS One, 2018, vol. 13, num. 7, p. e200696 |
URI: | http://hdl.handle.net/2445/130524 |
Related resource: | https://doi.org/10.1371/journal.pone.0200696 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
685166.pdf | 858.67 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License